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ID 111009
Title Alternative
年齢に応じた血中活性型ビタミンD濃度, 腎Cyp27b1およびCyp24a1発現に対する食餌性リン反応性の変化には腎α-Klotho遺伝子発現が関連する
Author
Yoshikawa, Ryouhei University of Tokushima
Yamamoto, Hironori University of Tokushima|Jin-ai University|University of Fukui KAKEN Search Researchers
Nakahashi, Otoki University of Tokushima|Tokushima Bunri University
Kagawa, Tomohiro University of Tokushima
Tajiri, Mari University of Tokushima
Nakao, Mari University of Tokushima
Fukuda, Shiori University of Tokushima
Arai, Hidekazu The University of Shizuoka
Iwano, Masayuki University of Fukui
Keywords
α-Klotho
age-related change
vitamin D metabolism
inorganic phosphate
mice
Content Type
Thesis or Dissertation
Description
In this study, we investigated the relationship between age-related changes in renal α-Klotho gene expression, vitamin D metabolism and the responsiveness of dietary phosphate in 1, 2 and 13 month-old mice fed a high phosphate (phosphate 1.2%) diet or low phosphate (phosphate 0.02%) diet for 5 days. We found that 1,25-dihydroxyvitamin D levels in plasma were significantly lower in the high phosphate group than the low phosphate group for 1 and 2 month-old mice, but not 13 month-old mice. In addition, in the high phosphate group plasma 1,25-dihydroxyvitamin D levels were decreased in 2 month-old mice relative to 1 month-old mice, but 13 month-old mice had higher levels than 2 month-old mice. In fact, plasma 1,25-dihydroxyvitamin D levels showed a significant correlation with vitamin D metabolism gene Cyp27b1 and Cyp24a1 mRNA expression in the high phosphate group. Interestingly, renal α-Klotho mRNA and protein levels were significant change with age. Furthermore, α-Klotho mRNA expression showed a significant negative correlation with plasma 1,25-dihydroxyvitamin D levels in the high phosphate group. Our results suggest that age-related alterations in renal α-Klotho expression could affect the responsiveness of dietary phosphate to vitamin D metabolism.
Journal Title
Journal of Clinical Biochemistry and Nutrition
ISSN
18805086
09120009
NCID
AA10710201
AA12091064
Publisher
SOCIETY FOR FREE RADICAL RESEARCH JAPAN
Volume
62
Issue
1
Start Page
68
End Page
74
Published Date
2018-01-01
Remark
内容要旨・審査要旨・論文本文の公開
本論文は, 著者Ryouhei Yoshikawaの学位論文として提出され, 学位審査・授与の対象となっている。
Rights
© 2018 Society for Free Radical Research Japan
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3119号
Diploma Number
甲栄第245号
Granted Date
2017-10-26
Degree Name
Doctor of Nutritional Science
Grantor
Tokushima University
departments
Medical Sciences