Effect of high-fat diet on phosphorus absorption
Kawamoto, Keisuke University of Shizuoka
Sakuma, Masae University of Shizuoka|Sugiyama Jogakuen University
Tanaka, Sarasa University of Hyogo
Masuda, Masashi University of Tokushima Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Nakao-Muraoka, Mari University of Tokushima
Niida, Yuki University of Tokushima
Nakamatsu, Yurino University of Shizuoka
Ito, Mikiko University of Hyogo
Taketani, Yutaka University of Tokushima Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Arai, Hidekazu University of Shizuoka
Serum phosphorus levels
Objective: Dietary carbohydrate/fat ratio may affect phosphorus metabolism because both calcium and phosphorus are regulated by similar metabolic mechanisms, and a high-fat diet (HF) induces deleterious effects on the absorption of dietary calcium. We hypothesized that the HF induces an increase in phosphorus absorption; therefore, this study aimed to evaluate the effects of differences in the quantity and quality of dietary fat on phosphorus metabolism over the short and long term.
Research Methods & Procedures: Eighteen 8-week-old Sprague-Dawley male rats were fed an isocaloric diet containing varied carbohydrate/fat energy ratio and sources of fat (control diet [Control], HF, and high saturated-fat diet [HF-SFA]). At 3 days and 7 weeks after the allocation and initiation of the test diets, feces and urine were collected and used for phosphorus and calcium measurement.
Results: The fecal phosphorous concentration (F-Pi) was lower in the HF-SFA group than in the other two groups; however, the urine phosphorus concentration (U-Pi) was significantly higher in the HF-SFA group than the other two groups when the rats were fed over the short (p<0.01) and long term (p<0.01 vs Control group, p<0.05 vs HF group). There were no significant differences in type-IIa sodium-phosphate cotransporter (NaPi-2a) and type-IIc sodium-phosphate cotransporter (NaPi-2c) mRNA expression, which are renal phosphate transport-related genes; however, the expression of type-IIb sodium-phosphate cotransporter (NaPi-2b) and type-III sodium-phosphate cotransporter (Pit-1) mRNA in the duodenum was higher in the HF and HF-SFA groups than in the Control group (p<0.05), although there were no significant differences in these in the jejunum.
Conclusions: Our results indicated that HF, particularly HF-SFA, increases intestinal phosphate absorption compared with Control.
© 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
|DOI (Published Version)|
|URL ( Publisher's Version )|
nut_72_110694.pdf 755 KB