ID 110666
Author
Arimochi, Hideki Department of Bacteriology, The University of Tokushima School of Medicine Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Kinouchi, Takemi Department of Bacteriology, The University of Tokushima School of Medicine
Kataoka, Keiko Department of Bacteriology, The University of Tokushima School of Medicine Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Kuwahara, Tomomi Department of Bacteriology, The University of Tokushima School of Medicine
Ohnishi, Yoshinari Department of Bacteriology, The University of Tokushima School of Medicine Tokushima University Educator and Researcher Directory
Keywords
Salmonella typhimurium
1-nitropyrene
nitroreductase activity
DNA adduct
mutagenicity
Content Type
Journal Article
Description
1-Nitropyrene (1-NP) is a mutagenic nitro compound in the environment. We studied correlations between the mutagenicity of 1-NP for three strains of Salmonella typhimurium, the activity of bacterial nitroreductases and the amount of 1-NP-derived DNA adducts. Bacterial strains used in this study were S. typhimurium strains TA98, nitroreductase-less mutant TA98NR and YG1021 carrying a nitroreductase-producing plasmid. The mutagenicity of 1-NP was measured using the Ames assay, and the nitroreductase activities of these strains were assayed by quantification of 1-aminopyrene produced from 1-NP. The DNA adducts were measured by the 32P-postlabeling method. Among the three bacterial strains, strain YG1021 was the highest in mutagenicity of 1-NP, the nitroreductase activity and the DNA adduct level. However, S. typhimurium strain TA98NR had the lowest values of these three parameters. Nitroreductase activity, DNA adduct level and mutagenicity were strongly correlated with each other. These results indicate that bacterial nitroreductase plays an important role in forming the DNA adducts, and that the higher the adduct level the higher the level of mutagenicity.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
44
Issue
3-4
Start Page
193
End Page
198
Sort Key
193
Published Date
1998
EDB ID
FullText File
language
eng
TextVersion
Publisher
departments
Medical Sciences