ID 110797
Author
Mazaki, Masanori Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School
Kataoka, Keiko Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Kinouchi, Takemi Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School
Vinitketkumnuen, Usanee Department of Biochemistry, Faculty of Medicine, Chiang Mai University
Yamada, Masami Division of Genetics and Mutagenesis, Biological Safety Research Center, National Institute of Health Sciences
Nohmi, Takehiko Division of Genetics and Mutagenesis, Biological Safety Research Center, National Institute of Health Sciences
Kuwahara, Tomomi Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School
Akimoto, Shigeru Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School
Ohnishi, Yoshinari Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory
Keywords
caraway
MNNG
antimutagenicity
O6-methylguanine
Content Type
Journal Article
Description
To elucidate the mechanism of antimutagenicity of caraway, we examined the effects of caraway seed extract on N-methyl-N’-nitro-N-nitrosoguanidine (MNNG)-induced mutagenesis in DNA methyltransferase-deficient Salmonella typhimurium strains, O6-methylguanineDNA adduct formation, and thiol content in S. typhimurium cells. MNNG was highly mutagenic for ogt- strains YG7104(ogt- ada+) and YG7108 (ogt-ada-), and it showed slightly higher mutagenicity in strain YG7100 (ogt+ ada-) than in strains TA100 and TA1535. Hot water extract of caraway seeds inhibited MNNG-induced mutation only in the ogt+ strains. In the presence of caraway extract, O6-methylguanine DNA adducts in strain YG7100 were decreased in proportion to the decrease of MNNG-induced mutagenesis. Although MNNG is known to degrade in the presence of thiols to produce methyl cation which can react with DNA, caraway had no effect on cellular concentrations of acid-soluble thiols. These results indicate that caraway does not directly inactivate MNNG and that Ogt-O6-methylguanine-DNAmethyltransferasemay be involved in the antimutagenic activity of caraway.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
53
Issue
1-2
Start Page
123
End Page
133
Sort Key
123
Published Date
2006-02
Remark
p.123 所属表記に誤記あり(誤)3.・・・Biologica 1 Safety Research Center, ・・・→(正)3.・・・Biological Safety Research Center, ・・・
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Medical Sciences