| ID | 83933 |
| Author |
Iwahashi, Shuichi
Department of Digestive Surgery and Transplantation, Institute of Health Biosciences, the University of Tokushima Graduate School
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Ishibashi, Hiroki
Department of Digestive Surgery and Transplantation, Institute of Health Biosciences, the University of Tokushima Graduate School
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Utsunomiya, Tohru
Department of Digestive Surgery and Transplantation, Institute of Health Biosciences, the University of Tokushima Graduate School
Morine, Yuji
Department of Digestive Surgery and Transplantation, Institute of Health Biosciences, the University of Tokushima Graduate School
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Ochir, Lkhaguva Tovuu
Department of Digestive Surgery and Transplantation, Institute of Health Biosciences, the University of Tokushima Graduate School
Hanaoka, Jun
Department of Digestive Surgery and Transplantation, Institute of Health Biosciences, the University of Tokushima Graduate School
Mori, Hiroki
Department of Digestive Surgery and Transplantation, Institute of Health Biosciences, the University of Tokushima Graduate School
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Ikemoto, Tetsuya
Department of Digestive Surgery and Transplantation, Institute of Health Biosciences, the University of Tokushima Graduate School
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Imura, Satoru
Department of Digestive Surgery and Transplantation, Institute of Health Biosciences, the University of Tokushima Graduate School
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Shimada, Mitsuo
Department of Digestive Surgery and Transplantation, Institute of Health Biosciences, the University of Tokushima Graduate School
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|
| Keywords | pancreas cancer
cholangiocarcinoma
HDAC inhibitor
valproic acid
epigenetic regulation
|
| Content Type |
Journal Article
|
| Description | Background : Histone deacetylase (HDAC) is well known to be associated with
tumorigenesis through epigenetic regulation, and its inhibitors (HDACIs) induce differentiation and apoptosis of tumor cells. We examined the therapeutic effects of valproic acid (VPA, a HDACI) with a combination of 5-fluorouracil (5-FU) in vitro. Methods : A human pancreas cancer cell line (SUIT-2) and a cholangiocarcinoma cell line (HuCCT1) were used. Cell viabilities were evaluated by a cell proliferation assay. We determined the anticancer effects of VPA combined with 5-FU in these cell lines. Results : Pancreas cancer (SUIT-2) : No effect of 5-FU (1.0 μM) was observed, but 17% and 30% of proliferationinhibitory effects were recognized in a dose of 2.5 or 5.0 μM, respectively. Cell viability was only weakly reduced by VPA (0.5 mM). However, in combination of 5-FU (1.0 μM) with VPA (0.5 mM), 19% of inhibitory effect was observed. Cholangiocarcinoma (HuCCT1) : 5-FU (1.0 μM) did not suppress the cell viability, but 5-FU (2.5 μM) suppressed by 23%. VPA (0.5 mM) did not suppress the cell viability, while VPA (1.0 mM) weakly decreased it by 11%. Combination of 5-FU (1.0 μM) and VPA (0.5 mM) markedly reduced the cell viability by 30%. Conclusion : VPA augmented the anti-tumor effects of 5-FU in cancer cell lines. Therefore, a combination therapy of 5-FU plus VPA may be a promising therapeutic option for patients with pancreas cancer and cholangiocarcinoma. |
| Journal Title |
The journal of medical investigation : JMI
|
| ISSN | 13431420
|
| NCID | AA11166929
|
| Volume | 58
|
| Issue | 1-2
|
| Start Page | 106
|
| End Page | 109
|
| Sort Key | 106
|
| Published Date | 2011-02
|
| Remark | The journal of medical investigation : http://medical.med.tokushima-u.ac.jp/jmi/index.html
|
| EDB ID | |
| FullText File | |
| language |
eng
|
| departments |
University Hospital
Medical Sciences
|
