ID 113301
Author
Yoshida, Masaki University of Tokushima
Yin, Yunjie University of Tokushima
Hattori, Atsushi University of Tokushima
Zenitani, Tomoe University of Tokushima
Hara, Sayuri University of Tokushima
Yonemoto, Haruka University of Tokushima
Nakamura, Aki University of Tokushima
Nakano, Masayuki University of Tokushima
Teshigawara, Kiyoshi Tokushima University
Arai, Hidekazu The University of Shizuoka
Hosaka, Toshio Tokushima University
Keywords
Glycerol-3-phosphate acyltransferase
Promoter
Sterol regulatory element-binding protein-1
Upstream stimulating factor-1
Liver
Mouse
Content Type
Journal Article
Description
Glycerol-3-phosphate acyltransferase 1 (GPAT1) is a rate limiting enzyme in de novo glycerophospholipid synthesis. The murine GPAT1 promoter sequence (the “classical” sequence) was reported previously. However, the organization of this DNA sequence does not fully match the mouse genome sequences on NCBI/GenBank. Here we have identified net cis-acting promoter sequences for the mouse GPAT1 gene: promoter 1a which includes part of the classical sequence and the downstream promoter 1b. Promoter 1a facilitates transcription of two alternative GPAT1 transcript variants, GPAT1-V1 and V2, while promoter 1b produces a third transcript variant, GPAT1-V3. Upstream stimulating factor-1 (USF-1) controlled both promoters whereas sterol regulatory element-binding protein-1 (SREBP-1) exclusively regulated promoter 1a activity in vitro. Feeding increased GPAT1-V1 and V2, but not V3 mRNA levels in mouse liver. The obese condition of db/db mice did not alter the hepatic expression levels of any of the three GPAT1 variants. Feeding enhanced hepatic mRNA levels, intranuclear protein levels and promoter 1a-binding levels of SREBP-1, but not of USF-1. Thus, promoter 1a was exclusively activated by routine feeding in vivo. Our results indicate differential roles of the two promoters in the regulation of hepatic GPAT1 gene expression in mice.
Journal Title
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
ISSN
13881981
NCID
AA11522965
AA11300409
Publisher
Elsevier
Volume
1791
Issue
1
Start Page
39
End Page
52
Published Date
2008-10-17
Remark
本論文は,著者Masaki Yoshidaの学位論文として提出され,学位審査・授与の対象となっている。
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Medical Sciences
Oral Sciences