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ID 117279
Title Alternative
pruRおよびPA0065遺伝子はAutoinducer Analog-1(AIA-1)による緑膿菌の抗菌薬耐性の低減に関与する
Author
Pahlevi, Muhammad Reza Tokushima University
Keywords
antibiotic tolerance
Pseudomonas aeruginosa
autoinducer analog-1
pruR
PA0066-65-64
Content Type
Thesis or Dissertation
Description
Pseudomonas aeruginosa infection is considered a high-risk nosocomial infection and is very difficult to eradicate because of its tolerance to antibiotic treatment. A new compound, autoinducer analog-1 (AIA-1), has been demonstrated to reduce antibiotic tolerance, but its mechanisms remain unknown. This study aimed to investigate the mechanisms of AIA-1 in the antibiotic tolerance of P. aeruginosa. A transposon mutant library was constructed using miniTn5pro, and screening was performed to isolate high tolerant mutants upon exposure to biapenem and AIA-1. We constructed a deletion mutant and complementation strain of the genes detected in transposon insertion site determination, pruR and PA0066-65-64, and performed killing assays with antibiotics and AIA-1. Gene expression upon exposure to biapenem and AIA-1 and their relationship to stress response genes were analyzed. High antibiotic tolerance was observed in Tn5-pruR and Tn5-PA0065 transposon mutants and their deletion mutants, ΔpruR and ΔPA0066-65-64. Complemented strains of pruR and PA0066-65-64 with their respective deletion mutants exhibited suppressed antibiotic tolerance. It was determined that deletion of PA0066-65-64 increased rpoS expression, and PA0066-65-64 affects antibiotic tolerance via the rpoS pathway. Additionally, antibiotics and AIA-1 were found to inhibit pruR and PA0066-65-64. This study proposed that pruR and PA0066-65-64 are members of the antibiotic tolerance suppressors.
Journal Title
Antibiotics
ISSN
20796382
Publisher
MDPI
Volume
11
Issue
6
Start Page
773
Published Date
2022-06-06
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Muhammad Reza Pahleviの学位論文として提出され,学位審査・授与の対象となっている。
Rights
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3709号
Diploma Number
甲口第488号
Granted Date
2023-03-23
Degree Name
Doctor of Dental Science
Grantor
Tokushima University
departments
Oral Sciences
Institute of Advanced Medical Sciences