ID 112166
Author
Inoue, Tomomi Tokushima University
Kinoshita, Maika Tokushima University
Oyama, Keisuke Sakai City Medical Center
Keywords
Captan
Intracellular Ca2+
Intracellular Zn2+
Nonprotein thiols
Superoxide anions
Lymphocytes
Content Type
Journal Article
Description
Captan, a phthalimide fungicide, is considered to be relatively nontoxic to mammals. There is a possibility that captan affects membrane and cellular parameters of mammalian cells, resulting in adverse effects, because of high residue levels. To test the possibility, we examined the effects of captan on rat thymic lymphocytes using flow-cytometry with appropriate fluorescent probes. Treatment with 10 and 30 μM captan induced apoptotic and necrotic cell death. Before cell death occurred, captan elevated the intracellular concentrations of Ca2+ and Zn2+ and decreased the concentration of cellular thiol compounds. These captan-induced phenomena are shown to cause cell death and are similar to those caused by oxidative stress. Captan also elevated the cytotoxicity of hydrogen peroxide. Results indicate that 10 and 30 μM captan cause cytotoxic effects on mammalian cells. Despite no report on the significant environmental toxicity hazard of captan in humans, it may exhibit adverse effects, described above, on wild organisms.
Journal Title
Environmental Toxicology and Pharmacology
ISSN
13826689
NCID
AA1107880X
AA11526671
Publisher
Elsevier
Volume
63
Start Page
78
End Page
83
Published Date
2018-08-27
Rights
© 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Author
departments
Bioscience and Bioindustry