Kamezaki, Chihiro Kyoto Pharmaceutical University
Nakashima, Ami Kyoto Pharmaceutical University
Yamada, Asako Kyoto Pharmaceutical University
Uenishi, Sachiko Kyoto Pharmaceutical University
Ishibashi, Hiroshi Kyoto Pharmaceutical University
Shibuya, Natsumi Department of Pharmaceutical Health Chemistry, Institute of Biomedical Sciences, Tokushima University, Graduate School
Hama, Susumu Kyoto Pharmaceutical University
Hosoi, Shinzo Kyoto Pharmaceutical University
Yamashita, Eiji AstaReal Co., Ltd.
Kogure, Kentaro Kyoto Pharmaceutical University|Department of Pharmaceutical Health Chemistry, Institute of Biomedical Sciences, Tokushima University, Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Astaxanthin and vitamin E are both effective antioxidants that are frequently used in cosmetics, as food additives, and in to prevent oxidative damage. A combination of astaxanthin and vitamin E would be expected to show an additive anntioxidative effect. In this study, liposomes co-encapsulating astaxanthin and the vitamin E derivatives α-tocopherol (α-T) or tocotrienols (T3) were prepared, and the antioxidative activity of these liposomes toward singlet oxygen and hydroxyl radical was evaluated in vitro. Liposomes co-encapsulating astaxanthin and α-T showed no additive anntioxidative effect, while the actual scavenging activity of liposomes co-encapsulating astaxanthin and T3 was higher than the calculated additive activity. To clarify why this synergistic effect occurs, the most stable structure of astaxanthin in the presence of α-T or α-T3 was calculated. Only α-T3 was predicted to form hydrogen bonding with astaxanthin, and the astaxanthin polyene chain would partially interact with the α-T3 triene chain, which could explain why there was a synergistic effect between astaxanthin and T3 but not α-T. In conclusion, co-encapsulation of astaxanthin and T3 induces synergistic scavenging activity by intermolecular interactions between the two antioxidants.
Journal of Clinical Biochemistry and Nutrition
Society for Free Radical Research Japan
© 2016 Society for Free Radical Research Japan
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