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ID 115720
Author
Hasan, Mahadi Kyoto Pharmaceutical University
Fujikawa, Koki Tokushima University
Ohgita, Takashi Kyoto Pharmaceutical University
Hama, Susumu Kyoto Pharmaceutical University
Saito, Hiroyuki Kyoto Pharmaceutical University KAKEN Search Researchers
Keywords
iRed
4′-thioDNA
RNAi effect
faint electric treatment
cytoplasmic delivery
Content Type
Journal Article
Description
An intelligent shRNA expression device (iRed) contains the minimum essential components needed for shRNA production in cells, and could be a novel tool to regulate target genes. However, general delivery carriers consisting of cationic polymers/lipids could impede function of a newly generated shRNA via electrostatic interaction in the cytoplasm. Recently, we found that faint electric treatment (fET) of cells enhanced delivery of siRNA and functional nucleic acids into the cytoplasm in the absence of delivery carriers. Here, we examined fET of cells stably expressing luciferase in the presence of iRed encoding anti-luciferase shRNA. Transfection of lipofectamine 2000 (LFN)/iRed lipoplexes showed an RNAi effect, but fET-mediated iRed transfection did not, likely because of the endosomal localization of iRed after delivery. However, fET in the presence of lysosomotropic agent chloroquine significantly improved the RNAi effect of iRed/fET to levels that were higher than those for the LFN/iRed lipoplexes. Furthermore, the amount of lipid droplets in adipocytes significantly decreased following fET with iRed against resistin in the presence of chloroquine. Thus, iRed could be a useful tool to regulate target genes following fET-mediated cytoplasmic delivery with endosomal escape devices.
Journal Title
Science and Technology of Advanced Materials
ISSN
14686996
18785514
NCID
AA11561821
AA11539797
Publisher
Taylor & Francis|National Institute for Materials Science
Volume
17
Issue
1
Start Page
554
End Page
562
Published Date
2016-09-16
Rights
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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DOI (Published Version)
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language
eng
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departments
Pharmaceutical Sciences