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ID 118028
Author
Michiue, Kohki Tokushima University
Takayama, Kentaro Kyoto Pharmaceutical University
Taniguchi, Atsuhiko Tokyo University of Pharmacy and Life Sciences
Hayashi, Yoshio Tokyo University of Pharmacy and Life Sciences
Keywords
sarcopenia
myostatin inhibitory peptides
iontophoresis
intramuscular deliver
peptide delivery
weak electricity
transdermal
Content Type
Journal Article
Description
Sarcopenia is a major public health issue that affects older adults. Myostatin inhibitory-D-peptide-35 (MID-35) can increase skeletal muscle and is a candidate therapeutic agent, but a non-invasive and accessible technology for the intramuscular delivery of MID-35 is required. Recently, we succeeded in the intradermal delivery of various macromolecules, such as siRNA and antibodies, by iontophoresis (ItP), a non-invasive transdermal drug delivery technology that uses weak electricity. Thus, we expected that ItP could deliver MID-35 non-invasively from the skin surface to skeletal muscle. In the present study, ItP was performed with a fluorescently labeled peptide on mouse hind leg skin. Fluorescent signal was observed in both skin and skeletal muscle. This result suggested that the peptide was effectively delivered to skeletal muscle from skin surface by ItP. Then, the effect of MID-35/ItP on skeletal muscle mass was evaluated. The skeletal muscle mass increased 1.25 times with ItP of MID-35. In addition, the percentage of new and mature muscle fibers tended to increase, and ItP delivery of MID-35 showed a tendency to induce alterations in the levels of mRNA of genes downstream of myostatin. In conclusion, ItP of myostatin inhibitory peptide is a potentially useful strategy for treating sarcopenia.
Journal Title
Pharmaceuticals
ISSN
14248247
Publisher
MDPI
Volume
16
Issue
3
Start Page
397
Published Date
2023-03-06
Rights
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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DOI (Published Version)
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language
eng
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departments
Pharmaceutical Sciences