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ID 116141
Title Alternative
ノビレチンは17型ヘルパーT細胞の分化調節により、実験的自己免疫性脳脊髄炎の発症を抑制する
Author
Hirabayashi, Yuwa Tokushima University
Anzaki, Chieri Tokushima University
Keywords
experimental autoimmune encephalomyelitis
nobiletin
polymethoxyflavonoid
Th17 differentiation
IL-17A
polymethoxyflavone
Content Type
Thesis or Dissertation
Description
Multiple sclerosis is an organ-specific autoimmune disease that targets the myelin antigen in the central nervous system. Nobiletin is a dietary polymethoxylated flavonoid found in citrus fruits. In this study, we investigated how nobiletin affects the disease state and immune responses to myelin oligodendrocyte glycoprotein in experimental autoimmune encephalomyelitis mice. Nobiletin was administered orally from 14 days before immunization until the end of the experiment, and clinical scores were determined. The production levels of interleukin-17A and interferon-γ were measured in a culture supernatant of splenocytes stimulated with myelin oligodendrocyte glycoprotein. In addition, flow cytometric analysis was performed to examine the effect of nobiletin on T cell differentiation in vitro. Admin­istration of nobiletin significantly decreased the clinical score and interleukin-17A production in splenocytes. Furthermore, in vitro analysis showed that nobiletin significantly suppressed Th17 cell differentiation and interleukin-17A production in a dose-dependent manner. The results suggest that nobiletin attenuates experimental autoimmune encephalomyelitis severity through modulation of Th17 cell differentiation.
Journal Title
Journal of Clinical Biochemistry and Nutrition
ISSN
09120009
18805086
NCID
AA10710201
Publisher
Society for Free Radical Research Japan
Volume
69
Issue
2
Start Page
145
End Page
150
Published Date
2021-09-01
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Akiko Nakamotoの学位論文として提出され,学位審査・授与の対象となっている。
Rights
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (https://creativecommons.org/licenses/by-nc-nd/4.0/).
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
乙第2117号
Diploma Number
乙栄第96号
Granted Date
2021-07-29
Degree Name
Doctor of Nutritional Science
Grantor
Tokushima University
departments
Medical Sciences