ID | 111886 |
Author |
Yoshida, Ichiro
Shikoku Junior College
Ito, Chihiro
Tokushima University
Matsuda, Shinya
Tokushima University
Tsuji, Akihiko
Tokushima University
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Yanaka, Noriyuki
Hiroshima University
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Keywords | melanogenesis
alisol B
Kampo
tyrosinase
microphthalmia-associated transcription factor (MITF)
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Content Type |
Journal Article
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Description | To develop new whitening agents from natural products, we screened 80 compounds derived from crude drugs in Kampo medicine in a melanin synthesis inhibition assay using murine B16 melanoma cells. The screen revealed that treatment with alisol B, a triterpene from Alismatis rhizoma, significantly decreased both melanin content and cellular tyrosinase activity in B16 cells. However, alisol B did not directly inhibit mushroom tyrosinase activity in vitro. Therefore, we investigated the mechanism underlying the inhibitory effect of alisol B on melanogenesis. Alisol B suppressed mRNA induction of tyrosinase and its transcription factor, microphthalmia-associated transcription factor (MITF). Furthermore, alisol B reduced the phosphorylation of CREB and maintained the activation of ERK1/2. These results suggest that the reduction in melanin production by alisol B is due to the downregulation of MITF through the suppression of CREB and activation of ERK, and that alisol B may be useful as a new whitening agent.
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Journal Title |
Bioscience, Biotechnology, and Biochemistry
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ISSN | 09168451
13476947
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NCID | AA10824164
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Publisher | Japan Society for Bioscience, Biotechnology, and Agrochemistry|Taylor & Francis
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Volume | 81
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Issue | 3
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Start Page | 534
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End Page | 540
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Published Date | 2017-01-04
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Remark | This is an electronic version of an article published in Bioscience, Biotechnology, and Biochemistry 81, 3, 534-540, 2017. Bioscience, Biotechnology, and Biochemistry is available online at: www.tandfonline.com/10.1080/09168451.2016.1268042.
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language |
eng
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Author
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departments |
Bioscience and Bioindustry
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