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ID 114608
Title Alternative
紫外線発光ダイオード照射は宿主細胞内でのウイルスRNAの複製と転写を抑制することでA型インフルエンザウイルスを不活化する
Author
Nishisaka-Nonaka, Risa Tokushima University
Yamamoto, Tomomi Tokushima University
Kojima, Mizuki Tokushima University
Wada, Takahiro Nihon Funen Co., Ltd.
Okamoto, Masayuki Nihon Funen Co., Ltd.
Ito, Hiroshi Nihon Funen Co., Ltd.
Yoshida, Ken-ichi Nihon Funen Co., Ltd.
Daidoji, Tomo Kyoto Prefectural University of Medicine
Nakaya, Takaaki Kyoto Prefectural University of Medicine
Keywords
Influenza A virus
ultraviolet
light-emitting diodes
UV-LED
Content Type
Thesis or Dissertation
Description
Influenza A viruses (IAVs) pose a serious global threat to humans and their livestock, especially poultry and pigs. This study aimed to investigate how to inactivate IAVs by using different ultraviolet-light-emitting diodes (UV-LEDs). We developed sterilization equipment with light-emitting diodes (LEDs) those peak wavelengths were 365 nm (UVA-LED), 310 nm (UVB-LED), and 280 nm (UVC-LED). These UV-LED irradiations decreased dose fluence-dependent plaque-forming units of IAV H1N1 subtype (A/Puerto Rico/8/1934) infected Madin-Darby canine kidney (MDCK) cells, but the inactivation efficiency of UVA-LED was significantly lower than UVB- and UVC-LED. UV-LED irradiations did not alter hemagglutination titer, but decreased accumulation of intracellular total viral RNA in infected MDCK cells was observed. Additionally, UV-LED irradiations suppressed the accumulation of intracellular mRNA (messenger RNA), vRNA (viral RNA), and cRNA (complementary RNA), as measured by strand-specific RT-PCR. These results suggest that UV-LEDs inhibit host cell replication and transcription of viral RNA. Both UVB- and UVC-LED irradiation decreased focus-forming unit (FFU) of H5N1 subtype (A/Crow/Kyoto/53/2004), a highly pathogenic avian IAV (HPAI), in infected MDCK cells, and the amount of FFU were lower than the H1N1 subtype. From these results, it appears that IAVs may have different sensitivity among the subtypes, and UVB- and UVC-LED may be suitable for HPAI virus inactivation.
Journal Title
Journal of Photochemistry and Photobiology B: Biology
ISSN
10111344
NCID
AA10686173
AA11533983
Publisher
Elsevier
Volume
189
Start Page
193
End Page
200
Published Date
2018-10-29
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Risa Nishisaka-Nonakaの学位論文として提出され,学位審査・授与の対象となっている。
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3415号
Diploma Number
甲栄第276号
Granted Date
2020-03-23
Degree Name
Doctor of Nutritional Science
Grantor
Tokushima University
departments
Medical Sciences
Bioscience and Bioindustry
Science and Technology