number of access : ?
number of downloads : ?
ID 110094
Title Alternative
顎関節におけるHIF-1αの役割
Author
Mino-Oka, Akiko Tokushima University
Shinohara, Takehiro Tokushima University
Tomita, Shuhei Osaka City University
Keywords
TMJ-OA
HIF-1α
Articular cartilage degradation
chondrocyte apoptosis
Content Type
Thesis or Dissertation
Description
Objective: Temporomandibular joint osteoarthritis (TMJ-OA) is a degenerative disease characterized by permanent cartilage loss. Articular cartilage is maintained in a low-oxygen environment. The chondrocyte response to hypoxic conditions involves expression of hypoxia inducible factor 1α (HIF-1α), which induces chondrocytes to increase expression of vascular endothelial growth factor (VEGF). Here, we investigated the role of HIF-1α in mechanical load effects on condylar cartilage and subchondral bone in heterozygous HIF-1α-deficient mice (HIF-1α+/-).
Design: Mechanical stress was applied to the TMJ of C57BL/6NCr wild-type (WT) and HIF-1α+/- mice with a sliding plate for 10 days. Histological analysis was performed by HE staining, Safranin-O/Fast green staining, and immunostaining specific for articular cartilage homeostasis.
Results: HIF-1α+/- mice had thinner cartilage and smaller areas of proteoglycan than WT controls, without and with mechanical stress. Mechanical stress resulted in prominent degenerative changes with increased expression of HIF-1α, VEGF, and the apoptosis factor cleaved Caspase-3 in condylar cartilage.
Conclusion: Our results indicate that HIF-1a may be important for articular cartilage homeostasis and protective against articular cartilage degradation in the TMJ under mechanical stress condition, therefore HIF-1α could be an important new therapeutic target in TMJ-OA.
Journal Title
Archives of Oral Biology
ISSN
00039969
NCID
AA00548457
AA11521918
Publisher
Elsevier
Volume
73
Start Page
274
End Page
281
Published Date
2016-10-28
Remark
内容要旨・審査要旨・論文本文の公開:
内容要旨:LID201704251016.pdf
審査要旨:LID201704251017.pdf
論文本文:LID201704251018.pdf
本論文は, 著者Akiko Mino-Okaの学位論文として提出され, 学位審査・授与の対象となっている。
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3072号
Diploma Number
甲口第415号
Granted Date
2017-03-23
Degree Name
Doctor of Dental Science
Grantor
Tokushima University
departments
Oral Sciences