| ID | 109488 |
| Title Alternative | ヒストンデメチラーゼJmjd3は転写因子Runx2とOsterixを介して骨芽細胞の分化を調節する
Regulation of osteoblast differentiation by Jmjd3
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| Author |
Yang, Di
The University of Tokushima|China Medical University
Haneji, Tatsuji
The University of Tokushima
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| Keywords | Jmjd3
H3K27me3
BMP-2
Osteoblast differentiation
Histone demethylation
Runx2
Osterix
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| Content Type |
Thesis or Dissertation
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| Description | Post-translational modifications of histones including methylation play important roles in cell differentiation. Jumonji domain-containing 3 (Jmjd3) is a histone demethylase, which specifically catalyzes the removal of trimethylation of histone H3 at lysine 27 (H3K27me3). In this study, we examined the expression of Jmjd3 in osteoblasts and its roles in osteoblast differentiation. Jmjd3 expression in the nucleus was induced in response to the stimulation of osteoblast differentiation as well as treatment of bone morphogenetic protein-2 (BMP-2). Either treatment with Noggin, an inhibitor of BMP-2, or silencing of Smad1/5, suppressed Jmjd3 expression during osteoblast differentiation. Silencing of Jmjd3 expression suppressed osteoblast differentiation through the expression of bone-related genes including Runx2, Osterix, Osteopontin, Bone sialoprotein (BSP), and Osteocalcin (OCN). Silencing of Jmjd3 decreased the promoter activities of Runx2 and Osterix and increased the level of H3K27me3 on the promoter regions of Runx2 and Osterix. Introduction of the exogenous Runx2 and Osterix partly rescued osteoblast differentiation in the shJmjd3 cells. The present results indicate that Jmjd3 plays important roles in osteoblast differentiation and regulates the expressions of BSP and OCN via transcription factors Runx2 and Osterix.
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| Journal Title |
The Journal of Biological Chemistry
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| Volume | 288
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| Issue | 47
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| Start Page | 33530
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| End Page | 33541
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| Published Date | 2013-10-8
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| Remark | 内容要旨・審査要旨・論文本文の公開:
内容要旨 : LID201509181013.pdf 審査要旨 : LID201509181014.pdf 論文本文 : LID201509181015.pdf 本論文は, 著者Di Yangの学位論文として提出され, 学位審査・授与の対象となっている。 |
| Rights | Copyright : The American Society for Biochemistry and Molecular Biology.
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| EDB ID | |
| DOI (Published Version) | |
| URL ( Publisher's Version ) | |
| FullText File | |
| language |
eng
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| TextVersion |
ETD
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| MEXT report number | 甲第2862号
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| Diploma Number | 甲口第401号
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| Granted Date | 2015-09-16
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| Degree Name |
Doctor of Dental Science
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| Grantor |
Tokushima University
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| departments |
Oral Sciences
Bioscience and Bioindustry
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