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ID 116182
Author
Yoshida, Kayo Tokushima University
Ono, Kisho Okayama University
Kawai, Hotaka Okayama University
Guo, Jiajie China Medical University|Okayama University
Wang, Ziyi Okayama University
Weng, Yao Okayama University
Yu, Yaqiong China Medical University|Okayama University
Uchida-Fukuhara, Yoko Okayama University
Ikegame, Mika Okayama University
Sasaki, Akira Okayama University
Nagatsuka, Hitoshi Okayama University
Kamioka, Hiroshi Okayama University
Keywords
Periodontal diseases
Lung diseases
Infection
Inflammation
Epithelial cells
Animal experimentation
Content Type
Journal Article
Description
Periodontal diseases are common inflammatory diseases that are induced by infection with periodontal bacteria such as Porphyromonas gingivalis (Pg). The association between periodontal diseases and many types of systemic diseases has been demonstrated; the term “periodontal medicine” is used to describe how periodontal infection/inflammation may impact extraoral health. However, the molecular mechanisms by which the factors produced in the oral cavity reach multiple distant organs and impact general health have not been elucidated. Extracellular vesicles (EVs) are nano-sized spherical structures secreted by various types of cells into the tissue microenvironment, and influence pathophysiological conditions by delivering their cargo. However, a detailed understanding of the effect of EVs on periodontal medicine is lacking. In this study, we investigated whether EVs derived from Pg-infected macrophages reach distant organs in mice and influence the pathophysiological status. EVs were isolated from human macrophages, THP-1 cells, infected with Pg. We observed that EVs from Pg-infected THP-1 cells (Pg-inf EVs) contained abundant core histone proteins such as histone H3 and translocated to the lungs, liver, and kidneys of mice. Pg-inf EVs also induced pulmonary injury, including edema, vascular congestion, inflammation, and collagen deposition causing alveoli destruction. The Pg-inf EVs or the recombinant histone H3 activated the NF-κB pathway, leading to increase in the levels of pro-inflammatory cytokines in human lung epithelial A549 cells. Our results suggest a possible mechanism by which EVs produced in periodontal diseases contribute to the progression of periodontal medicine.
Journal Title
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease
ISSN
09254439
NCID
AA10779707
AA11522998
Publisher
Elsevier
Volume
1867
Issue
11
Start Page
166236
Published Date
2021-08-11
Remark
論文本文は2022-08-11以降公開予定
Rights
© 2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
language
eng
TextVersion
その他
departments
Oral Sciences