Ohmoto, Yasukazu Otsuka Pharmaceutical Co.
Ogushi, Fumitaka The University of Tokushima
Muraguchi, Masahiro Otsuka Pharmaceutical Co.
Yamakawa, Mayumi Japan Red Cross Blood Center
Sone, Saburo The University of Tokushima Tokushima University Educator and Researcher Directory KAKEN Search Researchers
IL-1α receptor assay
Although autoantibodies to interleukin-1α (IL-1α autoantibodies) are known to be present in sera of apparently healthy humans, their frequency of occurrence and significance are unclear. To determine the prevalence of detectable IL-1α autoantibodies in normal human blood, we screened the plasma of blood donors (6290 subjects : 3977 men and 2313 women, ages 16 to 64 yr) by a radioimmunoassay which we developed using a method that could detect over 5 ng/ml. Moreover, we investigated immunoglobulin class of IL-1α autoantibodies and also their function. IL-1α autoantibodies were detected in 14.6% of the 6290 donors. Their frequency was higher in males than females (16.6% vs.11.2%, p<0.01) and increased with age in both sexes. The proportion of subjects with a high IL-1α autoantibodies titers also increased with age. We showed that IL-1α autoantibodies were of the IgG class and that they had neutralizing function to IL-1α by receptor assay. Neutralizing activity was only shown in plasma with concentration of IL-1α autoantibodies, the level of which was over 1000 ng/ml. The affinity of the IL-1α autoantibodies in plasma was between 2.1 X 10-10and 1.2 X 10-9 M (mean 6.4 X 10-10M). Our results provide a basis for comparison with IL-1α autoantibodies prevalence between healthy states and disease states, and suggest that IL-1α autoantibodies may play a significant role in modulating the effects of excessive IL-1α at local site or in systemic regions.
The Journal of Medical Investigation
The University of Tokushima School of Medicine
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