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ID 117793
Title Alternative
Fine structure of OPCs observed by SBF-SEM
Author
Ono, Katsuhiko Kyoto Prefectural University of Medicine
Gotoh, Hitoshi Kyoto Prefectural University of Medicine
Nomura, Tadashi Kyoto Prefectural University of Medicine
Matsumoto, Mami National Institute for Physiological Sciences|Nagoya City University
Saitoh, Sei National Institute for Physiological Sciences|Fujita Health University
Ohno, Nobuhiko Jichi Medical University|National Institute for Physiological Sciences
Content Type
Journal Article
Description
Oligodendrocyte precursor cells (OPC) arise from restricted regions of the central nervous system (CNS) and differentiate into myelin-forming cells after migration, but their ultrastructural characteristics have not been fully elucidated. This study examined the three-dimensional ultrastructure of OPCs in comparison with other glial cells in the early postnatal optic nerve by serial block-face scanning electron microscopy. We examined 70 putative OPCs (pOPC) that were distinct from other glial cells according to established morphological criteria. The pOPCs were unipolar in shape with relatively few processes, and their Golgi apparatus were localized in the perinuclear region with a single cisterna. Astrocytes abundant in the optic nerve were distinct from pOPCs and had a greater number of processes and more complicated Golgi apparatus morphology. All pOPCs and astrocytes contained a pair of centrioles (basal bodies). Among them, 45% of pOPCs extended a short cilium, and 20% of pOPCs had centrioles accompanied by vesicles, whereas all astrocytes with basal bodies had cilia with invaginated ciliary pockets. These results suggest that the fine structures of pOPCs during the developing and immature stages may account for their distinct behavior. Additionally, the vesicular transport of the centrioles, along with a short cilium length, suggests active ciliogenesis in pOPCs.
Journal Title
PLOS ONE
ISSN
19326203
Publisher
PLOS
Volume
17
Issue
12
Start Page
e0278118
Published Date
2022-12-01
Rights
This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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DOI (Published Version)
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FullText File
language
eng
TextVersion
Publisher
departments
Oral Sciences