Establishment of Immunodeficient Retinal Degeneration Model Mice and Functional Maturation of Human ESC-Derived Retinal Sheets after Transplantation

Iraha, Satoshi; Tu, Hung-Ya; Yamasaki, Suguru; Kagawa, Takahiro; Goto, Motohito; Takahashi, Riichi; Watanabe, Takehito; Sugita, Sunao; Yonemura, Shigenobu; Sunagawa, Genshiro A.; Matsuyama, Take; Fujii, Momo; Kuwahara, Atsushi; Kishino, Akiyoshi; Koide, Naoshi; Eiraku, Mototsugu; Tanihara, Hidenobu; Takahashi, Masayo; Mandai, Michiko

doi:10.1016/j.stemcr.2018.01.032

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Use this link to cite this item : https://repo.lib.tokushima-u.ac.jp/115734

ID	115734
Author	Iraha, Satoshi RIKEN\|Kumamoto University\|Kyoto University Tu, Hung-Ya RIKEN Yamasaki, Suguru RIKEN\|Sumitomo Dainippon Pharma Kagawa, Takahiro Animal Resources and Technical Research Center Goto, Motohito Animal Resources and Technical Research Center Takahashi, Riichi Animal Resources and Technical Research Center Watanabe, Takehito RIKEN Sugita, Sunao RIKEN Yonemura, Shigenobu RIKEN\|Tokushima University Tokushima University Educator and Researcher Directory KAKEN Search Researchers Sunagawa, Genshiro A. RIKEN Matsuyama, Take RIKEN Fujii, Momo RIKEN Kuwahara, Atsushi Sumitomo Dainippon Pharma Kishino, Akiyoshi Sumitomo Dainippon Pharma Koide, Naoshi RIKEN Eiraku, Mototsugu RIKEN Tanihara, Hidenobu Kumamoto University Takahashi, Masayo RIKEN\|Kyoto University Mandai, Michiko RIKEN
Content Type	Journal Article
Description	Increasing demand for clinical retinal degeneration therapies featuring human ESC/iPSC-derived retinal tissue and cells warrants proof-of-concept studies. Here, we established two mouse models of end-stage retinal degeneration with immunodeficiency, NOG-rd1-2J and NOG-rd10, and characterized disease progress and immunodeficient status. We also transplanted human ESC-derived retinal sheets into NOG-rd1-2J and confirmed their long-term survival and maturation of the structured graft photoreceptor layer, without rejection or tumorigenesis. We recorded light responses from the host ganglion cells using a multi-electrode array system; this result was consistent with whole-mount immunostaining suggestive of host-graft synapse formation at the responding sites. This study demonstrates an application of our mouse models and provides a proof of concept for the clinical use of human ESC-derived retinal sheets.
Journal Title	Stem Cell Reports
ISSN	22136711
Publisher	International Society for Stem Cell Research\|Cell Press
Volume	10
Issue	3
Start Page	1059
End Page	1074
Published Date	2018-03-01
Rights	This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
EDB ID	355254
DOI (Published Version)	10.1016/j.stemcr.2018.01.032
URL ( Publisher's Version )	https://doi.org/10.1016/j.stemcr.2018.01.032
FullText File	stemcr_10_3_1059.pdf 7.16 MB
language	eng
TextVersion	Publisher
departments	Medical Sciences