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ID 116075
Title Alternative
Chlorogenic acid supplementation during in vitro maturation improves maturation, fertilisation, and developmental competence of porcine oocytes
Effects of chlorogenic acid on porcine oocytes
Author
Nguyen, Thanh-Van Tokushima University
Do, Lanh Thi Kim Tokushima University|Yamaguchi University
Sato, Yoko University of East Asia
Taniguchi, Masayasu Yamaguchi University
Takagi, Mitsuhiro Yamaguchi University
Nguyen, Thanh Van Vietnam National University of Agriculture
Keywords
antioxidative stress
embryo
DNA fragmentation
chlorogenic acid
developmental competence
Content Type
Journal Article
Description
Chlorogenic acid (CGA) is a quinic acid conjugate of caffeic acid, and a phytochemical found in many fruits and beverages that acts as an antioxidant. The present study investigated the effects of CGA supplementation during in vitro maturation, on in vitro development of porcine oocytes, in order to improve the porcine in vitro production (IVP) system. Oocytes were matured either without (control) or with CGA (10, 50, 100, and 200 µM). Subsequently, the matured oocytes were fertilised, and cultured in vitro for 7 d. The rates of maturation, fertilisation, and blastocyst formation of oocytes matured with 50 µM CGA was significantly (p < 0.05) higher than those of the control oocytes. Hydrogen peroxide (H2O2) is one of the reactive oxygen species and induces DNA damage in porcine oocytes. When oocytes were matured with 1 mM H2O2 to assess the protective effect of CGA, 50 µM CGA supplementation improved the maturation rate and the proportion of DNA-fragmented nuclei in oocytes compared with control oocytes matured without CGA. Moreover, when oocytes were matured with either 50 µM CGA (control) or caffeic acid (10, 50, and 100 µM), the rates of maturation, fertilisation, and the blastocyst formation of oocytes matured with 50 µM CGA were similar to those of oocytes matured with 10 and 50 µM caffeic acid. Our results suggest that CGA has comparable effects to caffeic acid, and in vitro maturation with 50 µM CGA is particularly beneficial to in vitro production of porcine embryos and protects oocytes from DNA damage induced by oxidative stress. Supplementation of CGA to the maturation medium has a potential to improve porcine IVP system.
Journal Title
Reproduction in Domestic Animals
ISSN
14390531
Publisher
Wiley-VCH
Volume
52
Issue
6
Start Page
969
End Page
975
Published Date
2017-06-28
Remark
This is the peer reviewed version of the following article: Nguyen, T-V, Tanihara, F, Do, LTK et al. Chlorogenic acid supplementation during in vitro maturation improves maturation, fertilization and developmental competence of porcine oocytes. Reprod Dom Anim. 2017; 52: 969– 975., which has been published in final form at https://doi.org/10.1111/rda.13005. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
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DOI (Published Version)
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language
eng
TextVersion
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departments
Bioscience and Bioindustry