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ID 116079
Title Alternative
Epigenetic changes in histone H3 modification of Cat-Cow interspecies cloned embryos after treatment with the histone deacetylase inhibitor Trichostatin A
Histone H3 modification of iSCNT cat-cow embryos after TSA treatment
HISTONE H3 MODIFICATION OF ISCNT EMBRYOS
Author
Wittayarat, Manita Yamaguchi University|Chulalongkorn University
Sato, Yoko Yamaguchi University|University of East Asia
Do, Lanh Thi Kim Yamaguchi University
Chatdarong, Kaywalee Chulalongkorn University
Tharasanit, Theerawat Chulalongkorn University
Techakumphu, Mongkol Chulalongkorn University
Taniguchi, Masayasu Yamaguchi University
Keywords
acetylation
iSCNT
mammal
methylation
reprogramming
cat
cow
histone
interspecies nuclear transfer
Trichostatin A
Content Type
Journal Article
Description
This study aimed to determine the acetylation patterns on histone H3K9/18/23 and the dimethylation pattern on histone H3K9 during early embryogenesis among 50 nM Trichostatin A (TSA)-treated iSCNT cat-cow embryos, untreated iSCNT cat-cow embryos (control) and bovine in vitro fertilisation (IVF) embryos, because TSA-treated iSCNT embryos are able to develop into blastocysts. The results show that the acetylation levels of H3K9/18/23 in the TSA-treated iSCNT and bovine IVF embryos were higher than those in the control embryos at almost all of the examined stages (2 h post-fusion / post-insemination (PF/PI), pronuclear (PN), two-cell, four-cell and eight-cell stages). At 6 h PF/PI the acetylation levels on H3K9/23 in the TSA-treated iSCNT and bovine IVF embryos were lower than those in the control, and there was no difference in the acetylation levels of H3K18 among the three groups. The acetylation levels of H3K9/23 increased either in the TSA-treated iSCNT or and bovine IVF embryos increased when those embryos developed to the PN and two-cell stages. The dimethylation level of H3K9 in the TSA-treated iSCNT embryos resembled that of the bovine IVF embryos at all examined stages (2h PF/PI, 6 h PF/PI and PN stages), and these levels were greater than those of the control. This result suggests that treatment of iSCNT embryos with TSA modifies the patterns of histone acetylation and dimethylation at certain lysine residues in a manner that is comparable with that seen in IVF embryos during early embryogenesis.
Journal Title
Animal Science Journal
ISSN
17400929
NCID
AA11677214
Publisher
Japanese Society of Animal Science|Wiley
Volume
88
Issue
4
Start Page
593
End Page
601
Published Date
2016-08-09
Remark
This is the peer reviewed version of the following article: Wittayarat, M., Sato, Y., Do, L. T. K., Chatdarong, K., Tharasanit, T., Techakumphu, M., Taniguchi, M., and Otoi, T. (2017) Epigenetic modulation on cat-cow interspecies somatic cell nuclear transfer embryos by treatment with trichostatin A. Anim Sci J, 88: 593– 601., which has been published in final form at https://doi.org/10.1111/asj.12676. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Author
departments
Bioscience and Bioindustry