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ID 114216
Title Alternative
Stat5 determines the development of mammary alveolar cells
Author
Shillingford, Jonathan M. National Institutes of Health
Smith, Gilbert H. National Institutes of Health
Grimm, Sandra L. Baylor College of Medicine
Wagner, Kay-Uwe National Institutes of Health|University of Nebraska Medical Center
Oka, Takami National Institutes of Health
Rosen, Jeffrey M. Baylor College of Medicine
Robinson, Gertraud W. National Institutes of Health
Hennighausen, Lothar National Institutes of Health
Content Type
Journal Article
Description
Functional development of mammary epithelium during pregnancy depends on prolactin signaling. However, the underlying molecular and cellular events are not fully understood. We examined the specific contributions of the prolactin receptor (PrlR) and the signal transducers and activators of transcription 5a and 5b (referred to as Stat5) in the formation and differentiation of mammary alveolar epithelium. PrlR- and Stat5-null mammary epithelia were transplanted into wild-type hosts, and pregnancy-mediated development was investigated at a histological and molecular level. Stat5-null mammary epithelium developed ducts but failed to form alveoli, and no milk protein gene expression was observed. In contrast, PrlR-null epithelium formed alveoli-like structures with small open lumina. Electron microscopy revealed undifferentiated features of organelles and a perturbation of cell–cell contacts in PrlR- and Stat5-null epithelia. Expression of NKCC1, an Na-K-Cl cotransporter characteristic for ductal epithelia, and ZO-1, a protein associated with tight junction, were maintained in the alveoli-like structures of PrlR- and Stat5-null epithelia. In contrast, the Na-Pi cotransporter Npt2b, and the gap junction component connexin 32, usually expressed in secretory epithelia, were undetectable in PrlR- and Stat5- null mice. These data demonstrate that signaling via the PrlR and Stat5 is critical for the proliferation and differentiation of mammary alveoli during pregnancy.
Journal Title
Journal of Cell Biology
ISSN
00219525
15408140
NCID
AA00694812
AA1203766X
Publisher
Rockefeller University Press
Volume
155
Issue
4
Start Page
531
End Page
542
Published Date
2001-11-12
Rights
© 2001 Keiko Miyoshi et al. Originally published in Journal of Cell Biology. https://doi.org/10.1083/jcb.200107065
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Oral Sciences