ID | 117551 |
Author |
Hara, Hideyuki
Tokushima University
Tokushima University Educator and Researcher Directory
KAKEN Search Researchers
Chida, Junji
Tokushima University
Tokushima University Educator and Researcher Directory
KAKEN Search Researchers
Pasiana, Agriani Dini
Tokushima University
Imamura, Morikazu
University of Miyazaki
Mori, Tsuyoshi
University of Miyazaki
Takatsuki, Hanae
University of Miyazaki
Atarashi, Ryuichiro
University of Miyazaki
|
Keywords | prions
prion protein
protein misfolding
neurodegeneration
ethanolamine
therapy
|
Content Type |
Journal Article
|
Description | Prion diseases are a group of fatal neurodegenerative disorders caused by accumulation of proteinaceous infectious particles, or prions, which mainly consist of the abnormally folded, amyloidogenic prion protein, designated PrPSc. PrPSc is produced through conformational conversion of the cellular isoform of prion protein, PrPC, in the brain. To date, no effective therapies for prion diseases have been developed. In this study, we incidentally noticed that mouse neuroblastoma N2a cells persistently infected with 22L scrapie prions, termed N2aC24L1-3 cells, reduced PrPSc levels when cultured in advanced Dulbecco’s modified eagle medium (DMEM) but not in classic DMEM. PrPC levels remained unchanged in prion-uninfected parent N2aC24 cells cultured in advanced DMEM. These results suggest that advanced DMEM may contain an anti-prion compound(s). We then successfully identified ethanolamine in advanced DMEM has an anti-prion activity. Ethanolamine reduced PrPSc levels in N2aC24L1-3 cells, but not PrPC levels in N2aC24 cells. Also, oral administration of ethanolamine through drinking water delayed prion disease in mice intracerebrally inoculated with RML scrapie prions. These results suggest that ethanolamine could be a new anti-prion compound.
|
Journal Title |
International Journal of Molecular Sciences
|
ISSN | 14220067
|
Publisher | MDPI
|
Volume | 22
|
Issue | 21
|
Start Page | 11742
|
Published Date | 2021-10-29
|
Rights | This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
|
EDB ID | |
DOI (Published Version) | |
URL ( Publisher's Version ) | |
FullText File | |
language |
eng
|
TextVersion |
Publisher
|
departments |
Institute of Advanced Medical Sciences
|