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ID 114734
Title Alternative
単球由来線維細胞のパラクライン効果を介した線維化促進作用の機能解析
Fibrocytes activate fibroblasts by PDGF.
Author
Abe, Shuichi Tokushima University
Kishi, Masami Tokushima University
Takahashi, Naoki Tokushima University
Keywords
fibrocyte
fibroblast
idiopathic pulmonary fibrosis
PDGF
Content Type
Thesis or Dissertation
Description
Fibrocytes, which are bone marrow-derived collagen-producing cells, were reported to play a role in the pathogenesis of pulmonary fibrosis. However, their function in pulmonary fibrosis is unclear. We analyzed their function compared with that of monocytes and localization in fibrotic tissues in patients with idiopathic pulmonary fibrosis (IPF). We compared the gene expression profile of monocyte-derived fibrocytes with that of monocytes by microarray analysis. Proliferation and differentiation into myofibroblasts were examined by 3H-thymidine incorporation assay and Western blotting. We measured the level of growth factors in the culture supernatant of fibrocytes by ELISA. The localization of fibrocytes in lung tissues of patients with IPF was determined by immunofluorescence staining. Compared with monocytes, fibrocytes had higher expression of extracellular matrix- and growth factor-encoding genes, including PDGF-B, FGF-2 and VEGF-B. Although fibrocytes did not proliferate in response to PDGF, co-culture of fibrocytes stimulated the growth of lung fibroblasts through the production of PDGF-BB. In the lung of IPF patients, CD45+Collagen-I+FSP-1+ cells, which have a similar phenotype to fibrocytes, were detected and co-stained with anti-PDGF antibody. This study suggested that fibrocytes function in pulmonary fibrosis partly by producing PDGF in the lungs of IPF patients.
Journal Title
The Journal of Medical Investigation
ISSN
13496867
13431420
NCID
AA12022913
AA11166929
Publisher
Tokushima University Faculty of Medicine
Volume
67
Issue
1-2
Start Page
102
End Page
112
Sort Key
102
Published Date
2020-02
Remark
内容要旨・審査要旨・論文本文の公開
本論文は, 著者Shuichi Abeの学位論文として提出され, 学位審査・授与の対象となっている。
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3363号
Diploma Number
甲医第1446号
Granted Date
2020-03-05
Degree Name
Doctor of Medical Science
Grantor
Tokushima University
departments
University Hospital
Medical Sciences