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ID 116252
Author
Oniki, Kentaro Kumamoto University
Nohara, Hirofumi Kumamoto University
Nakashima, Ryunosuke Kumamoto University
Obata, Yui Kumamoto University
Muto, Narumi Kumamoto University
Sakamoto, Yuki Kumamoto University
Ueno-Shuto, Keiko Sojo University
Imafuku, Tadashi Kumamoto University
Watanabe, Hiroshi Kumamoto University
Maruyama, Toru Kumamoto University
Otake, Koji Japanese Red Cross Kumamoto Health Care Center
Ogata, Yasuhiro Japanese Red Cross Kumamoto Health Care Center
Suico, Mary Ann Kumamoto University
Kai, Hirofumi Kumamoto University
Shuto, Tsuyoshi Kumamoto University
Saruwatari, Junji Kumamoto University
Content Type
Journal Article
Description
Oxidative stress and inflammation play a key role in the age-related decline in the respiratory function. Adipokine in relation to the metabolic and inflammatory systems is attracting growing interest in the field of respiratory dysfunction. The present clinical and experimental studies investigated the role of the disulfide bond-forming oxidoreductase A-like protein (DsbA-L) gene, which has antioxidant and adiponectin multimeric (i.e. activation) properties, on the respiratory function of the elderly. We performed a retrospective longitudinal genotype-phenotype relationship analysis of 318 Japanese relatively elderly participants (mean age ± standard deviation: 67.0 ± 5.8 years) during a health screening program and an in vitro DsbA-L knock-down evaluation using 16HBE14o-cells, a commonly evaluated human airway epithelial cell line. The DsbA-L rs1917760 polymorphism was associated with a reduction in the ratio of forced expiratory volume in 1 second (FEV1)/forced vital capacity (FVC) and %FEV1 and with the elevation of the prevalence of FEV1/FVC < 70%. We also confirmed that the polymorphism was associated with a decreased respiratory function in relation to a decrease in the ratio of high-molecular-weight adiponectin/total adiponectin (as a marker of adiponectin multimerization) and an increase in the oxidized human serum albumin (as an oxidative stress marker). Furthermore, we clarified that DsbA-L knock-down induced oxidative stress and up-regulated the mucus production in human airway epithelial cells. These findings suggest that the DsbA-L gene may play a role in protecting the respiratory function of the elderly, possibly via increased systemic adiponectin functions secreted from adipocytes or through systemic and/or local pulmonary antioxidant properties.
Journal Title
Scientific Reports
ISSN
20452322
Publisher
Springer Nature
Volume
10
Start Page
5973
Published Date
2020-04-06
Rights
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
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language
eng
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departments
Pharmaceutical Sciences