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ID 114563
Title Alternative
Specificity of iron-phytosiderophore transporter
Author
Harada, Erisa Suntory Foundation for Life Sciences
Sugase, Kenji Suntory Foundation for Life Sciences|Kyoto University
Murata, Yoshiko Suntory Foundation for Life Sciences
Keywords
α-helical content
phytosiderophore
YS1 transporter
Content Type
Journal Article
Description
Hordeum vulgare L. yellow stripe 1 (HvYS1) is a selective transporter of Fe(III)-phytosiderophores in barley that is responsible for iron acquisition from the soil. In contrast, maize Zea mays, yellow stripe 1 (ZmYS1) possesses broad substrate specificity. In this study, a quantitative evaluation of the transport activities of HvYS1 and ZmYS1 chimera proteins revealed that the seventh extracellular membrane loop is essential for substrate specificity. The loop peptides of both transporters were prepared and analysed by circular dichroism and NMR. The spectra revealed a higher propensity for α-helical conformation of the HvYS1 loop peptide and a largely disordered structure for that of ZmYS1. These structural differences are potentially responsible for the substrate specificities of the transporters.
Journal Title
FEBS Letters
ISSN
18733468
NCID
AA00642943
Publisher
Federation of European Biochemical Societies|Wiley
Volume
590
Issue
24
Start Page
4617
End Page
4627
Published Date
2016-11-11
Rights
This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
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DOI (Published Version)
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language
eng
TextVersion
Publisher
departments
Pharmaceutical Sciences