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ID 119004
Author
Kikuchi, Takeshi Otsuka Pharmaceutical Factory, Inc.
Nishimura, Masuhiro Otsuka Pharmaceutical Factory, Inc.
Shirakawa, Chikage Otsuka Pharmaceutical Factory, Inc.
Fujita, Yasutaka Otsuka Pharmaceutical Factory, Inc.
Keywords
Human adipose tissuee–derived
mesenchymal stem cell
Cell preservation solution
Cell aggregation
Oxygen partial pressure
Content Type
Journal Article
Description
Introduction: This study investigated the storage conditions under which cell aggregation occurs and the conditions that inhibit cell aggregation when human adipose tissue-derived mesenchymal stem cells (hADSCs) are stored in lactated Ringer’s solution (LR) supplemented with 3% trehalose and 5% dextran 40 (LR-3T-5D).
Methods: We first examined the effects of storage temperature and time on the aggregation and viability of hADSCs stored in LR and LR-3T-5D. The cells were stored at 5 °C or 25 °C for various times up to 24 h. We then evaluated the effects of storage volume (250-2,000 μL), cell density (2.5-20 × 105 cells/mL), and nitrogen gas replacement on aggregation, oxygen partial pressure (pO2), and viability of hADSCs stored for 24 h at 25 °C in LR-3T-5D.
Results: When stored in LR-3T-5D, viability did not change under either condition compared with pre-storage, but the cell aggregation rate increased significantly with storage at 25 °C for 24 h (p<0.001). In LR, the aggregation rate did not change under either condition, but cell viability decreased significantly after 24 h at both 5 °C and 25 °C (p < 0.05). The cell aggregation rates and pO2 tended to decrease with increasing solution volume and cell density. Nitrogen gas replacement significantly decreased the cell aggregation rate and pO2 (p < 0.05). However, there were no differences in viability among cells stored under conditions of different storage volumes, densities, and nitrogen gas replacement.
Conclusions: Aggregation of cells after storage at 25 °C in LR-3T-5D may be suppressed by increasing the storage volume and cell density as well as by incorporating nitrogen replacement, which lowers the pO2 in the solution.
Journal Title
Regenerative Therapy
ISSN
23523204
Publisher
Japanese Society of Regenerative Medicine|Elsevier
Volume
24
Start Page
25
End Page
31
Published Date
2023-06-01
Rights
The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
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DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Bioscience and Bioindustry