ID | 114154 |
Title Alternative | Epigallocatechin 3-gallateのNrf2経路を介したマウス膵島細胞保護作用に関する検討
protective effect of EGCG on mouse islets
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Author |
Takata, Atsushi
Tokushima University
Wada, Yuma
Tokushima University
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Ikemoto, Tetsuya
Tokushima University
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Morine, Yuji
Tokushima University
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Saito, Yu
Tokushima University
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Keywords | Epigallocatechin 3-gallate (EGCG)
Nrf2
islet transplantation
Reactive oxygen species (ROS)
Pre-conditioning
Nuclear factor erythroid 2-related factor 2 (Nrf2)
Reactive oxygen species (ROS) production
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Content Type |
Thesis or Dissertation
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Description | Purpose: Epigallocatechin 3-gallate (EGCG), a green tea polyphenol, has been shown to have anti-oxidant and anti-inflammatory effects in vitro and in vivo. The aim of this study was to investigate the effects and mechanism of EGCG on isolated pancreatic islets as pre-conditioning for pancreatic islet transplantation.
Methods: The pancreatic islets were divided into two groups: an islet culture medium group (control) and an islet culture medium with EGCG (100 μM) group. We investigated the islet viability, Nrf2 expression, reactive oxygen species (ROS) production, and heme oxygenase-1 (HO-1) mRNA. Five hundred islet equivalents after 12 h of culture for the EGCG 100 μM and control group were transplanted under the kidney capsule of streptozotocin (STZ)-induced diabetic ICR mice. Results: The cell viability and insulin secretion ability in the EGCG group were preserved, and the nuclear translocation of Nrf2 was increased in the EGCG group (p<0.01). While the HO-1 mRNA levels were also higher in the EGCG group than in the control group (p<0.05), the ROS production was lower (p<0.01). An in vivo functional assessment showed that the blood glucose level had decreased in the EGCG group after transplantation (p<0.01). Conclusion: EGCG protects the viability and function of islets by suppressing ROS production via the Nrf2 pathway. |
Journal Title |
Surgery Today
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ISSN | 09411291
14362813
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NCID | AA10824685
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Publisher | Springer|Japan Surgical Society
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Volume | 49
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Issue | 6
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Start Page | 536
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End Page | 545
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Published Date | 2019-02-07
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Remark | 内容要旨・審査要旨・論文本文の公開
本論文は,著者Atsushi Takataの学位論文として提出され,学位審査・授与の対象となっている。 This is a post-peer-review, pre-copyedit version of an article published in Surgery Today. The final authenticated version is available online at: https://doi.org/10.1007/s00595-019-1761-0 |
EDB ID | |
DOI (Published Version) | |
URL ( Publisher's Version ) | |
FullText File | |
language |
eng
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TextVersion |
ETD
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MEXT report number | 甲第3342号
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Diploma Number | 甲医第1434号
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Granted Date | 2019-12-26
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Degree Name |
Doctor of Medical Science
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Grantor |
Tokushima University
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departments |
University Hospital
Medical Sciences
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