ID | 116998 |
Title Alternative | CRISPR-Cas9システムを用いた味覚受容体発現調節物質のスクリーニング系の開発
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Author |
Beppu, Kana
Tokushima University
Tsutsumi, Rie
Tokushima University
Tokushima University Educator and Researcher Directory
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Ansai, Satoshi
Tohoku University
Ochiai, Nana
Tokushima University
Terakawa, Mai
Tokushima University
Mori, Marie
Tokushima University
Horikawa, Kazuki
Tokushima University
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Tomoi, Takumi
National Institute for Basic Biology
Sakamoto, Joe
National Institute for Basic Biology
Kamei, Yasuhiro
National Institute for Basic Biology|The Graduate University for Advanced Studies (SOKENDAI)
Naruse, Kiyoshi
The Graduate University for Advanced Studies (SOKENDAI)|National Institute for Basic Biology
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Keywords | taste 1 receptor 3
dysgeusia
medaka (Oryzias latipes)
CRISPR-Cas9 system
monosodium glutamate
5-fluorouracil
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Content Type |
Thesis or Dissertation
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Description | Taste recognition mediated by taste receptors is critical for the survival of animals in nature and is an important determinant of nutritional status and quality of life in humans. However, many factors including aging, diabetes, zinc deficiency, infection with influenza or cold viruses, and chemotherapy can trigger dysgeusia, for which a standard treatment has not been established. We here established an engineered strain of medaka (Oryzias latipes) that expresses green fluorescent protein (GFP) from the endogenous taste 1 receptor 3 (T1R3) gene locus with the use of the CRISPR-Cas9 system. This T1R3-GFP knock-in (KI) strain allows direct visualization of expression from this locus by monitoring of GFP fluorescence. The pattern of GFP expression in the T1R3-GFP KI fish thus mimicked that of endogenous T1R3 gene expression. Furthermore, exposure of T1R3-GFP KI medaka to water containing monosodium glutamate or the anticancer agent 5-fluorouracil resulted in an increase or decrease, respectively, in GFP fluorescence intensity, effects that also recapitulated those on T1R3 mRNA abundance. Finally, screening for agents that affect GFP fluorescence intensity in T1R3-GFP KI medaka identified tryptophan as an amino acid that increases T1R3 gene expression. The establishment of this screening system for taste receptor expression in medaka provides a new tool for the development of potential therapeutic agents for dysgeusia.
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Journal Title |
Biochemical and Biophysical Research Communications
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ISSN | 0006291X
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NCID | AA00564395
AA11542044
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Publisher | Elsevier
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Volume | 601
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Start Page | 65
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End Page | 72
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Published Date | 2022-02-23
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Remark | 内容要旨・審査要旨・論文本文の公開
本論文は,著者Kana Beppuの学位論文として提出され,学位審査・授与の対象となっている。 |
EDB ID | |
DOI (Published Version) | |
URL ( Publisher's Version ) | |
FullText File | |
language |
eng
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TextVersion |
ETD
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MEXT report number | 甲第3600号
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Diploma Number | 甲栄第288号
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Granted Date | 2022-03-23
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Degree Name |
Doctor of Nutritional Science
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Grantor |
Tokushima University
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departments |
Medical Sciences
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