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ID 116998
Title Alternative
CRISPR-Cas9システムを用いた味覚受容体発現調節物質のスクリーニング系の開発
Author
Beppu, Kana Tokushima University
Ansai, Satoshi Tohoku University
Ochiai, Nana Tokushima University
Terakawa, Mai Tokushima University
Mori, Marie Tokushima University
Tomoi, Takumi National Institute for Basic Biology
Sakamoto, Joe National Institute for Basic Biology
Kamei, Yasuhiro National Institute for Basic Biology|The Graduate University for Advanced Studies (SOKENDAI)
Naruse, Kiyoshi The Graduate University for Advanced Studies (SOKENDAI)|National Institute for Basic Biology
Keywords
taste 1 receptor 3
dysgeusia
medaka (Oryzias latipes)
CRISPR-Cas9 system
monosodium glutamate
5-fluorouracil
Content Type
Thesis or Dissertation
Description
Taste recognition mediated by taste receptors is critical for the survival of animals in nature and is an important determinant of nutritional status and quality of life in humans. However, many factors including aging, diabetes, zinc deficiency, infection with influenza or cold viruses, and chemotherapy can trigger dysgeusia, for which a standard treatment has not been established. We here established an engineered strain of medaka (Oryzias latipes) that expresses green fluorescent protein (GFP) from the endogenous taste 1 receptor 3 (T1R3) gene locus with the use of the CRISPR-Cas9 system. This T1R3-GFP knock-in (KI) strain allows direct visualization of expression from this locus by monitoring of GFP fluorescence. The pattern of GFP expression in the T1R3-GFP KI fish thus mimicked that of endogenous T1R3 gene expression. Furthermore, exposure of T1R3-GFP KI medaka to water containing monosodium glutamate or the anticancer agent 5-fluorouracil resulted in an increase or decrease, respectively, in GFP fluorescence intensity, effects that also recapitulated those on T1R3 mRNA abundance. Finally, screening for agents that affect GFP fluorescence intensity in T1R3-GFP KI medaka identified tryptophan as an amino acid that increases T1R3 gene expression. The establishment of this screening system for taste receptor expression in medaka provides a new tool for the development of potential therapeutic agents for dysgeusia.
Journal Title
Biochemical and Biophysical Research Communications
ISSN
0006291X
NCID
AA00564395
AA11542044
Publisher
Elsevier
Volume
601
Start Page
65
End Page
72
Published Date
2022-02-23
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Kana Beppuの学位論文として提出され,学位審査・授与の対象となっている。
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3600号
Diploma Number
甲栄第288号
Granted Date
2022-03-23
Degree Name
Doctor of Nutritional Science
Grantor
Tokushima University
departments
Medical Sciences