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ID 113340
Title Alternative
Epigallocatechin-3-gallateは大腸癌細胞株においてNrf2とオートファジーを介して放射線感受性を増強する
EGCG Enhances Radiation Sensitivity in Colorectal Cancer Cells
Author
Enkhbat, Tumenjin Tokushima University
Keywords
Epigallocatechin-3-gallate
Radiation
Colorectal cancer
Nuclear factor (erythroid-derived 2)-like 2
Autophagy
Content Type
Thesis or Dissertation
Description
Background/Aim: Epigallocatechin-3-gallate (EGCG) is a major polyphenolic component of green tea. EGCG plays a potential role in radio-sensitizing cancer cells. The combined effect of EGCG and radiation was investigated in a colorectal cancer cell line, focusing on nuclear factor (erythroid-derived 2)-like 2 (Nrf2) autophagy signalling. Materials and Methods: HCT-116 cells were treated with 12.5 μM EGCG for different periods of time, 2 Gy radiation, or both. Cell viability was determined with the WST-8 assay. The number of colonies was determined with the colony formation assay. mRNA expression of LC3 and caspase-9 was analyzed with quantitative real-time polymerase chain reaction. Results: Combination treatment with EGCG and radiation significantly decreased the growth of HCT-116 cells. The number of colonies was reduced to 34.2% compared to the control group. Immunofluorescence microscopy images showed that nuclear translocation of Nrf2 was significantly increased when cells were treated with the combination of EGCG and radiation compared to the control and single-treatment groups. Combined treatment with EGCG and radiation significantly induced LC3 and caspase-9 mRNA expression. Conclusion: EGCG increased the sensitivity of colorectal cancer cells to radiation by inhibiting cell proliferation and inducing Nrf2 nuclear translocation and autophagy.
Journal Title
Anticancer Research
ISSN
02507005
17917530
NCID
AA10625860
AA12440673
Publisher
The International Institute of Anticancer Research
Volume
38
Issue
11
Start Page
6247
End Page
6252
Published Date
2018-11-05
Remark
内容要旨・審査要旨・論文本文の公開
本論文は, 著者TUMENJIN ENKHBATの学位論文として提出され, 学位審査・授与の対象となっている。
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3256号
Diploma Number
甲医第1405号
Granted Date
2019-03-22
Degree Name
Doctor of Medical Science
Grantor
Tokushima University
departments
Medical Sciences
University Hospital