ID | 114608 |
Title Alternative | 紫外線発光ダイオード照射は宿主細胞内でのウイルスRNAの複製と転写を抑制することでA型インフルエンザウイルスを不活化する
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Author |
Nishisaka-Nonaka, Risa
Tokushima University
Mawatari, Kazuaki
Tokushima University
Tokushima University Educator and Researcher Directory
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Yamamoto, Tomomi
Tokushima University
Kojima, Mizuki
Tokushima University
Uebanso, Takashi
Tokushima University
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Nakahashi, Mutsumi
Tokushima University
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Emoto, Takahiro
Tokushima University
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Akutagawa, Masatake
Tokushima University
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Kinouchi, Yohsuke
Tokushima University
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Wada, Takahiro
Nihon Funen Co., Ltd.
Okamoto, Masayuki
Nihon Funen Co., Ltd.
Ito, Hiroshi
Nihon Funen Co., Ltd.
Yoshida, Ken-ichi
Nihon Funen Co., Ltd.
Daidoji, Tomo
Kyoto Prefectural University of Medicine
Nakaya, Takaaki
Kyoto Prefectural University of Medicine
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Keywords | Influenza A virus
ultraviolet
light-emitting diodes
UV-LED
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Content Type |
Thesis or Dissertation
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Description | Influenza A viruses (IAVs) pose a serious global threat to humans and their livestock, especially poultry and pigs. This study aimed to investigate how to inactivate IAVs by using different ultraviolet-light-emitting diodes (UV-LEDs). We developed sterilization equipment with light-emitting diodes (LEDs) those peak wavelengths were 365 nm (UVA-LED), 310 nm (UVB-LED), and 280 nm (UVC-LED). These UV-LED irradiations decreased dose fluence-dependent plaque-forming units of IAV H1N1 subtype (A/Puerto Rico/8/1934) infected Madin-Darby canine kidney (MDCK) cells, but the inactivation efficiency of UVA-LED was significantly lower than UVB- and UVC-LED. UV-LED irradiations did not alter hemagglutination titer, but decreased accumulation of intracellular total viral RNA in infected MDCK cells was observed. Additionally, UV-LED irradiations suppressed the accumulation of intracellular mRNA (messenger RNA), vRNA (viral RNA), and cRNA (complementary RNA), as measured by strand-specific RT-PCR. These results suggest that UV-LEDs inhibit host cell replication and transcription of viral RNA. Both UVB- and UVC-LED irradiation decreased focus-forming unit (FFU) of H5N1 subtype (A/Crow/Kyoto/53/2004), a highly pathogenic avian IAV (HPAI), in infected MDCK cells, and the amount of FFU were lower than the H1N1 subtype. From these results, it appears that IAVs may have different sensitivity among the subtypes, and UVB- and UVC-LED may be suitable for HPAI virus inactivation.
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Journal Title |
Journal of Photochemistry and Photobiology B: Biology
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ISSN | 10111344
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NCID | AA10686173
AA11533983
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Publisher | Elsevier
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Volume | 189
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Start Page | 193
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End Page | 200
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Published Date | 2018-10-29
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Remark | 内容要旨・審査要旨・論文本文の公開
本論文は,著者Risa Nishisaka-Nonakaの学位論文として提出され,学位審査・授与の対象となっている。 |
DOI (Published Version) | |
URL ( Publisher's Version ) | |
FullText File | |
language |
eng
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TextVersion |
ETD
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MEXT report number | 甲第3415号
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Diploma Number | 甲栄第276号
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Granted Date | 2020-03-23
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Degree Name |
Doctor of Nutritional Science
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Grantor |
Tokushima University
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departments |
Medical Sciences
Bioscience and Bioindustry
Science and Technology
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