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ID 117004
Title Alternative
紫外線発光ダイオードの照射は、そのピーク波長によって異なるウイルス分子を標的にすることで単純ヘルペスウイルスの感染性を減弱する
Author
Bui, Thi Kim Ngan Tokushima University
Fukushima, Shiho Tokushima University
Shimohata, Takaaki Tokushima University|Fukui Prefectural University KAKEN Search Researchers
Keywords
UV-LED
herpes simplex virus type 1
DNA photoproducs
viral lipids
Light-emitting diode
Ultraviolet
Content Type
Thesis or Dissertation
Description
Herpes simplex virus type 1 (HSV-1) is an enveloped virus that mainly infects humans. Given its high global prevalence, disinfection is critical for reducing the risk of infection. Ultraviolet-light-emitting diodes (UV-LEDs) are eco-friendly irradiating modules with different peak wavelengths, but the molecules degraded by UV-LED irradiation have not been clarified. To identify the target viral molecules of UV-LEDs, we exposed HSV-1 suspensions to UV-LED irradiation at wavelengths of 260-, 280-, 310-, and 365-nm and measured viral DNA, protein, and lipid damage and infectivity in host cells. All UV-LEDs substantially reduced by inhibiting host cell transcription, but 260- and 280-nm UV-LEDs had significantly stronger virucidal efficiency than 310- and 365-nm UV-LEDs. Meanwhile, 260- and 280-nm UV-LEDs induced the formation of viral DNA photoproducts and the degradation of viral proteins and some phosphoglycerolipid species. Unlike 260- and 280-nm UV-LEDs, 310- and 365-nm UV-LEDs decreased the viral protein levels, but they did not drastically change the levels of viral DNA photoproducts and lipophilic metabolites. These results suggest that UV-LEDs reduce the infectivity of HSV-1 by targeting different viral molecules based on the peak wavelength. These findings could facilitate the optimization of UV-LED irradiation for viral inactivation.
Journal Title
Journal of Photochemistry and Photobiology B: Biology
ISSN
10111344
NCID
AA10686173
AA11533983
Publisher
Elsevier
Volume
228
Start Page
112410
Published Date
2022-02-14
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Thi Kim Ngan Buiの学位論文として提出され,学位審査・授与の対象となっている。
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3602号
Diploma Number
甲栄第290号
Granted Date
2022-03-23
Degree Name
Doctor of Nutritional Science
Grantor
Tokushima University
departments
Medical Sciences
Science and Technology