ID | 115331 |
Title Alternative | 甲状腺未分化癌同所移植SCIDマウスモデルへのパクリタキセルとレンバチニブの有効性に対する小動物用FDG-PET/CTを用いた非侵襲的モニタリング
MONITORING ANAPLASTIC THYROID CANCER MODELS BY PET/CT
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Author |
Aoyama, Mariko
The University of Tokushima
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Takizawa, Hiromitsu
The University of Tokushima
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Otani, Tamaki
Tokushima University
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Bando, Yoshimi
Tokushima University
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Uehara, Hisanori
Tokushima University
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Kondo, Kazuya
The University of Tokushima
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Tangoku, Akira
The University of Tokushima
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Keywords | anaplastic thyroid carcinoma
orthotopic model
18F-FDG PET/CT
paclitaxel
lenvatinib
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Content Type |
Thesis or Dissertation
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Description | Anaplastic thyroid carcinoma (ATC) is a rare type of thyroid carcinoma with a poor prognosis. Thus, suitable preclinical tumor models are required for the development of new ATC therapies. In the present study, orthotopic tumor xenograft models were established using ATC cell lines and SCID mice, and tumor invasion and the effects of anticancer drugs were evaluated using positron emission tomography/computed tomography (PET/CT) to repeatedly and non-invasively monitor these models. Three ATC cell lines (8305c, 8505c, and ACT-1) were used. Their sensitivities to two anticancer drugs (paclitaxel and lenvatinib) were investigated. The 8505c cell line was orthotopically implanted into SCID mice, which were then divided into three groups: no chemotherapy, paclitaxel (5 mg/kg, administered intraperitoneally, every week), and lenvatinib (5 mg/kg, oral route, every day) groups. PET/CT was performed and tumor growth and the effects of anticancer drugs based on tumor volume and fludeoxyglucose (FDG) uptake were evaluated. 8505c cells exhibited the highest sensitivity to the anticancer drugs. In mice implanted with 8505c cells, continuous increases in FDG uptake associated with tumor growth were detected on PET/CT in the group that received no chemotherapy. The tumor volume and FDG uptake increased by 91.5- and 2.4-fold, respectively, within 2 weeks. The increase observed in tumor volume was 26.9- and 12.2-fold in the paclitaxel and lenvatinib groups, respectively, within 2 weeks. Furthermore, the increase in FDG uptake was 1.8-fold and 1.6-fold in the paclitaxel and lenvatinib groups, respectively, within 2 weeks. In our orthotopic SCID mouse model, tumor growth and the effects of anticancer drugs were repeatedly and non-invasively monitored using PET/CT. The present method is useful for the development of new ATC treatments.
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Journal Title |
Oncology Reports
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ISSN | 1021335X
17912431
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NCID | AA11016405
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Publisher | Spandidos Publications
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Volume | 44
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Issue | 4
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Start Page | 1709
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End Page | 1716
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Published Date | 2020-08-07
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Remark | 内容要旨・審査要旨・論文本文の公開
本論文は,著者Mariko Aoyamaの学位論文として提出され,学位審査・授与の対象となっている。 |
EDB ID | |
DOI (Published Version) | |
URL ( Publisher's Version ) | |
FullText File | |
language |
eng
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TextVersion |
ETD
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MEXT report number | 甲第3461号
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Diploma Number | 甲医第1468号
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Granted Date | 2020-09-24
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Degree Name |
Doctor of Medical Science
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Grantor |
Tokushima University
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departments |
Medical Sciences
Advance Radiation Research, Education, and Management Center
University Hospital
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