Total for the last 12 months
number of access : ?
number of downloads : ?
ID 118778
Title Alternative
OTUB1-YAP1経路の頭頸部扁平上皮癌の悪性化への関与
Author
Jin, Shengjian Tokushima University
Hagita, Hiroko Tokushima University
Sarubo, Motoharu Tokushima University
Qi, Guangying Guilin Medical University
Keywords
Deubiquitinating enzyme (DUB)
head and neck squamous cell carcinoma
invasion
OTUB1
YAP
Content Type
Thesis or Dissertation
Description
Background: Comprehending the molecular mechanisms underlying head and neck squamous cell carcinoma (HNSCC) is vital for the development of effective treatment strategies. Deubiquitinating enzymes (DUBs), which regulate ubiquitin-dependent pathways, are potential targets for cancer therapy because of their structural advantages. Here we aimed to identify a potential target for HNSCC treatment among DUBs.
Methods: A screening process was conducted using RNA sequencing data and clinical information from HNSCC patients in the TCGA database. A panel of 88 DUBs was analyzed to identify those associated with poor prognosis. Subsequently, HNSCC cells were modified to overexpress specific DUBs, and their effects on cell proliferation and invasion were evaluated. In vivo experiments were performed to validate the findings.
Results: In HNSCC patients, USP10, USP14, OTUB1, and STAMBP among the screened DUBs were associated with a poor prognosis. Among them, OTUB1 showed the most aggressive characteristics in both in vitro and in vivo experiments. Additionally, OTUB1 regulated the stability and nuclear localization of YAP1, a substrate involved in cell proliferation and invasion. Notably, OTUB1 expression exhibited a positive correlation with the HNSCC-YAP score in HNSCC cells.
Conclusions: This study highlights the critical role of OTUB1 in HNSCC progression via modulating YAP1. Targeting the OTUB1-YAP1 axis holds promise as a potential therapeutic strategy for HNSCC treatment.
Journal Title
Cancer Medicine
ISSN
20457634
Publisher
John Wiley & Sons
Volume
12
Issue
24
Start Page
22156
End Page
22169
Published Date
2023-11-20
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Shengjian Jinの学位論文として提出され,学位審査・授与の対象となっている。
Rights
This is an open access article under the terms of the Creative Commons Attribution License, (https://creativecommons.org/licenses/by/4.0/) which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3826号
Diploma Number
甲口第501号
Granted Date
2024-03-22
Degree Name
Doctor of Dental Science
Grantor
Tokushima University
departments
Medical Sciences
Oral Sciences