Total for the last 12 months
number of access : ?
number of downloads : ?
ID 113212
Title Alternative
サイトカイン誘発好中球化学誘引物質の発現はTNFα発現を抑制することにより卵胞の閉鎖およびアポトーシスから回避させる
Author
Tanaka, Yu Japanese Red Cross Kochi Hospital KAKEN Search Researchers
Kuwahara, Akira The University of Tokushima KAKEN Search Researchers
Ushigoe, Kenjiro The University of Tokushima
Yano, Yuya The University of Tokushima
Taniguchi, Yuka The University of Tokushima
Matsuzaki, Toshiya The University of Tokushima KAKEN Search Researchers
Keywords
Apoptosis
follicle
ovary
ovulation
tumor necrosis factor alpha
Content Type
Thesis or Dissertation
Description
Aim: Cytokine-induced neutrophil chemoattractant (CINC/gro) is a CXC family chemokine, similar to interleukin-8 in rats, and is one of the factors that regulates ovulation. However, the mechanism that regulates atresia of the ovaries postovulation is not clearly defined.
Methods: Whether antibody-blocking of CINC/gro can alter the number of ovulated oocytes and modulate neutrophil infiltration was investigated. The effect of the antibody on the level of inflammatory cytokine production and follicular atresia was examined. Apoptosis was measured by the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) method and via analysis of the messenger RNA expression of Bcl-2 and Bcl2-associated X (Bax).
Results: The anti-CINC/gro antibody treatment decreased the number of ovulated oocytes. The messenger RNA levels of cyclooxygenase-2 and interleukin-1 beta were decreased by the antibody treatment, whereas that of tumor necrosis factor (TNF) alpha was increased. The TUNEL analysis revealed a larger number of apoptotic cells in the antibody group, compared with those in the control group, as well as a significant increase in the Bax/Bcl-2 ratio 24 hours after human chorionic gonadotropin administration.
Conclusion: These findings suggest that ovulation is accelerated by neutrophil infiltration into the theca layer. The CINC/gro appears to synergize with interleukin-1 beta for ovulation. By contrast, the data suggest that CINC/gro expression suppresses TNF alpha expression and that CINC/gro expression therefore prevents the follicles from undergoing atresia and apoptosis.
Journal Title
Reproductive Medicine and Biology
ISSN
14470578
NCID
AA11706516
Publisher
Wiley|Japan Society for Reproductive Medicine
Volume
16
Issue
2
Start Page
157
End Page
165
Published Date
2017-03-12
Remark
内容要旨・審査要旨・論文本文の公開
本論文は, 著者Yu Tanakaの学位論文として提出され, 学位審査・授与の対象となっている。
Rights
This is an open access article under the terms of the Creative Commons Attribution License(https://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
© 2017 The Authors. Reproductive Medicine and Biology published by John Wiley & Sons Australia, Ltd on behalf of Japan Society for Reproductive Medicine.
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
乙第2107号
Diploma Number
乙医第1756号
Granted Date
2019-03-19
Degree Name
Doctor of Medical Science
Grantor
Tokushima University
departments
Medical Sciences
University Hospital