ID | 29070 |
Author |
Tanaka, Haruko
Third Department of Internal Medicine, The University of Tokushima School of Medicine
Maeda, Kenichi
Health Service Center, The University of Tokushima
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Nakamura, Yoichi
Third Department of Internal Medicine, The University of Tokushima School of Medicine
Azuma, Masahiko
Third Department of Internal Medicine, The University of Tokushima School of Medicine
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Yanagawa, Hiroaki
Third Department of Internal Medicine, The University of Tokushima School of Medicine
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Sone, Saburo
Third Department of Internal Medicine, The University of Tokushima School of Medicine
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Keywords | bronchial epithelial cells
APC
T cells
CD40
IFN-γ
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Content Type |
Journal Article
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Description | We examined whether freshly isolated human bronchial cells (HBEC) and bronchial epithelial cell line/ BEAS-2B cells expressed surface molecules required for APC function. These cells expressed CD40 and ICAM-1, but not B7-1, B7-2 or HLA-DR molecules. Treatment of these cells with IFN-γ resulted in enhanced expression of CD40 and ICAM-1 as well as induction of HLA-DR expression. Th2 cytokines such as IL-4 and IL-5, proinflammatory cytokine of GM-CSF and nonspecific activator endotoxin had no effect on these phenotypic expressions. Functional examinations showed that allogeneic lymphocytes purified from peripheral blood strongly proliferated in response to BEAS-2B cells cultured with IFN-γ, but only weakly compared with those without IFN-γ. When allogeneic lymphocytes were purified to CD4+ cells, the proliferative response against BEAS-2B cells was abolished. Blockade of CD40-CD40L interaction by anti-CD40 antibody also inhibited the proliferation of lymphocytes to BEAS-2B cells, although this treatment showed a minimum effect on the response to allogeneic MNC. Thus, bronchial epithelial cells have the ability to present allogeneic antigens to T cells in both CD40- and IFN-γ- dependent manners under the presence of third party cells that transduce co-stimulatory signals.
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Journal Title |
The journal of medical investigation : JMI
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ISSN | 13431420
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NCID | AA11166929
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Volume | 48
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Issue | 1-2
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Start Page | 109
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End Page | 117
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Sort Key | 109
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Published Date | 2001
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Remark | |
EDB ID | |
FullText File | |
language |
eng
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departments |
Health Service and Counseling Center
Medical Sciences
University Hospital
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