ID | 109370 |
Title Alternative | Cyclopamineは大腸癌癌幹細胞のソニック・ヘッジホッグとその下流遺伝子発現を抑制する
Sonic Hedgehog Downstream Genes in Cancer Stem Cells
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Author |
Batsaikhan, Bat-Erdene
The University of Tokushima
Iwata, Takashi
The University of Tokushima
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Takasu, Chie
The University of Tokushima
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Kashihara, Hideya
The University of Tokushima
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Shimada, Mitsuo
The University of Tokushima
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Keywords | Cancer stem-like cells
epithelial-mesenchymal transition
cancer cells
sonic hedgehog
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Content Type |
Thesis or Dissertation
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Description | Backround: Most solid cancers including colon cancer are believed to be initiated from and maintained by cancer stem cells (CSCs), that are responsible for treatment resistance, resulting in tumor relapse. The aim of this study was to clarify the possible role of the Sonic Hedgehog (Shh) signaling pathway in the regulation of cancer stem cells. Materials and Methods: The HCT-116 cell line was cultured with fetal bovine serum in RPMI-1640 medium and its sphere was grown in serum-free non-adherent culture. Gene expressions were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) from cells treated with and without cyclopamine. Results: HCT-116 sphere-derived cells grown in serum-free, non-adherent culture, showed significantly increased expression of stem cell markers, Shh downstream genes and epithelial-mesenchymal transition (EMT) markers compared to parental cells grown in conventional culture. The expression of stemness markers, Shh downstream genes and EMT markers were higher in cancer spheres than the parental cell line and down-regulated by cyclopamine treatment in a dose-dependent manner. Conclusion: Overall, these findings show that cyclopamine treatment could down-regulate the expression of stemness markers, shh downstream genes and EMT markers on HCT-116 spheres.
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Journal Title |
Anticancer Research
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ISSN | 02507005
17917530
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NCID | AA10625860
AA12440673
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Publisher | The International Institute of Anticancer Research
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Volume | 34
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Issue | 11
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Start Page | 6339
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End Page | 6344
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Published Date | 2014-11-03
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Remark | 内容要旨・審査要旨・論文本文の公開:
内容要旨・審査要旨 : LID201505251001.pdf 論文本文 : k2796_fulltext.pdf 本論文は,著者BAT-ERDENE BATSAIKHANの学位論文として提出され, 学位審査・授与の対象となっている。 著者の申請により要約(2015-05-25公開)に替えて論文全文を公開(2022-12-14) |
EDB ID | |
URL ( Publisher's Version ) | |
FullText File | |
language |
eng
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TextVersion |
ETD
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MEXT report number | 甲第2796号
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Diploma Number | 甲医第1238号
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Granted Date | 2015-03-23
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Degree Name |
Doctor of Medical Science
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Grantor |
Tokushima University
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departments |
University Hospital
Liberal Arts and Sciences
Medical Sciences
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