ID | 119311 |
Title Alternative | 偏光分解顕微鏡を用いたアルコール性肝線維症の線維化の量的・質的評価系の開発
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Author |
Matsuzaki, Saya
Tokushima University
Hase, Eiji
Tokushima University
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Takanari, Hiroki
Tokushima University|Nihon University
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Hayashi, Yuri
Tokushima University
Hayashi, Yusaku
Tokushima University
Oshikata, Haruto
Tokushima University
Minamikawa, Takeo
Tokushima University
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Kimura, Satoko
Tokyo Medical Examiner’s Office
Ichimura‑Shimizu, Mayuko
Tokushima University
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Yasui, Takeshi
Tokushima University
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Keywords | SHGimage
hepatic scleosis
collagen fiber
fatty liver disease
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Content Type |
Thesis or Dissertation
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Description | Liver fibrosis is assessed mainly by conventional staining or second harmonic generation (SHG) microscopy, which can only provide collagen content in fibrotic area. We propose to use polarization-resolved SHG (PR-SHG) microscopy to quantify liver fibrosis in terms of collagen fiber orientation and crystallization. Liver samples obtained from autopsy cases with fibrosis stage of F0–F4 were evaluated with an SHG microscope, and 12 consecutive PR-SHG images were acquired while changing the polarization azimuth angle of the irradiated laser from 0° to 165° in 15° increments using polarizer. The fiber orientation angle (φ) and degree (ρ) of collagen were estimated from the images. The SHG-positive area increased as the fibrosis stage progressed, which was well consistent with Sirius Red staining. The value of φ was random regardless of fibrosis stage. The mean value of ρ (ρ-mean), which represents collagen fiber crystallinity, varied more as fibrosis progressed to stage F3, and converged to a significantly higher value in F4 than in other stages. Spatial dispersion of ρ (ρ-entropy) also showed increased variation in the stage F3 and decreased variation in the stage F4. It was shown that PR-SHG could provide new information on the properties of collagen fibers in human liver fibrosis.
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Journal Title |
Scientific Reports
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ISSN | 20452322
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Publisher | Springer Nature
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Volume | 13
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Start Page | 22100
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Published Date | 2023-12-13
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Remark | 内容要旨・審査要旨・論文本文の公開
本論文は,著者Saya Matsuzakiの学位論文として提出され,学位審査・授与の対象となっている。 |
Rights | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
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EDB ID | |
DOI (Published Version) | |
URL ( Publisher's Version ) | |
FullText File | |
language |
eng
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TextVersion |
ETD
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MEXT report number | 甲第3785号
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Diploma Number | 甲医第1600号
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Granted Date | 2024-03-07
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Degree Name |
Doctor of Medical Science
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Grantor |
Tokushima University
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departments |
Institute of Post-LED Photonics
University Hospital
Science and Technology
Medical Sciences
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