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ID 119311
Title Alternative
偏光分解顕微鏡を用いたアルコール性肝線維症の線維化の量的・質的評価系の開発
Author
Matsuzaki, Saya Tokushima University
Hayashi, Yuri Tokushima University
Hayashi, Yusaku Tokushima University
Oshikata, Haruto Tokushima University
Kimura, Satoko Tokyo Medical Examiner’s Office
Keywords
SHGimage
hepatic scleosis
collagen fiber
fatty liver disease
Content Type
Thesis or Dissertation
Description
Liver fibrosis is assessed mainly by conventional staining or second harmonic generation (SHG) microscopy, which can only provide collagen content in fibrotic area. We propose to use polarization-resolved SHG (PR-SHG) microscopy to quantify liver fibrosis in terms of collagen fiber orientation and crystallization. Liver samples obtained from autopsy cases with fibrosis stage of F0–F4 were evaluated with an SHG microscope, and 12 consecutive PR-SHG images were acquired while changing the polarization azimuth angle of the irradiated laser from 0° to 165° in 15° increments using polarizer. The fiber orientation angle (φ) and degree (ρ) of collagen were estimated from the images. The SHG-positive area increased as the fibrosis stage progressed, which was well consistent with Sirius Red staining. The value of φ was random regardless of fibrosis stage. The mean value of ρ (ρ-mean), which represents collagen fiber crystallinity, varied more as fibrosis progressed to stage F3, and converged to a significantly higher value in F4 than in other stages. Spatial dispersion of ρ (ρ-entropy) also showed increased variation in the stage F3 and decreased variation in the stage F4. It was shown that PR-SHG could provide new information on the properties of collagen fibers in human liver fibrosis.
Journal Title
Scientific Reports
ISSN
20452322
Publisher
Springer Nature
Volume
13
Start Page
22100
Published Date
2023-12-13
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Saya Matsuzakiの学位論文として提出され,学位審査・授与の対象となっている。
Rights
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3785号
Diploma Number
甲医第1600号
Granted Date
2024-03-07
Degree Name
Doctor of Medical Science
Grantor
Tokushima University
departments
Institute of Post-LED Photonics
University Hospital
Science and Technology
Medical Sciences