New treatment strategies for uterine sarcoma using secreted frizzled‑related proteins

Secreted frizzled‑related proteins (SFRPs) are involved in the development of various types of cancer and function by suppressing the Wnt signaling pathway. To elucidate the clinical implications of SFRPs in uterine sarcoma, SFRP expression levels and their effects on uterine leiomyosarcoma cells were examined. Immunostaining for SFRP4 was performed on uterine smooth muscle, uterine fibroid and uterine leiomyosarcoma tissues. Additionally, the effects of SFRP4 administration on cell viability, migration and adhesion were evaluated in uterine leiomyosarcoma SKN cells using the WST‑1 assay (Roche Diagnostics) and the CytoSelect™ 24‑well Cell Migration Assay Kit and the CytoSelect™ 48‑well Cell Adhesion Assay Kit. The expression levels of SFRP4 in uterine leiomyosarcoma tissues were lower than those in normal smooth muscle and uterine fibroid tissues. In addition, SFRP4 suppressed the viability and migration, and increased the adhesion ability of uterine leiomyosarcoma cells compared with in the control group. In conclusion, SFRP4 may suppress the viability and migration, and enhance the adhesion of sarcoma cells. These results suggested that SFRP4 could be considered as a novel therapeutic target for uterine sarcoma.

内容要旨・審査要旨・論文本文の公開
本論文は，著者TOMOHIRO KAGAWAの学位論文として提出され，学位審査・授与の対象となっている。
Kagawa T, Mineda A, Nakagawa T, Shinohara A, Arakaki R, Inui H, Noguchi H, Yoshida A, Kinouchi R, Yamamoto Y, Yamamoto Y, et al: New treatment strategies for uterine sarcoma using secreted frizzled‑related proteins. Exp Ther Med 27: 231, 2024