ID | 114302 |
Title Alternative | 免疫欠損肺高血圧モデルマウスに対するヒト臍帯血由来単核球細胞移植の効果
Human cord blood-MNC transplantation improves PH
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Author |
Sugano, Mikio
Tokushima University
Tokushima University Educator and Researcher Directory
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Yoshida, Homare
Tokushima University
Kitaichi, Takashi
Tokushima University
Tokushima University Educator and Researcher Directory
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Sugasawa, Noriko
Tokushima University
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Keywords | Pulmonary hypertension
cellular therapy
umbilical cord blood
monocrotaline
mononuclear cells
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Content Type |
Thesis or Dissertation
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Description | Objectives : To investigate the effects of human umbilical cord blood-derived mononuclear cell (hUCB-MNC) transplantation on pulmonary hypertension (PH) induced by monocrotaline (MCT) in immunodeficient mice and their distribution. Methods :MCT was administered to BALB/c Slc-nu/nu mice, and PH was induced in mice 4 weeks later. Fresh hUCB-MNCs harvested from a human donor after her delivery were injected intravenously into those PH mice. The medial thickness of pulmonary arterioles, ratio of right ventricular to septum plus left ventricular weight (RV/S+LV), and ratio of acceleration time to ejection time of pulmonary blood flow waveform (AT/ET) were determined 4 weeks after hUCB-MNC transplantation. To reveal the incorporation into the lung, CMTMR-labeled hUCB-MNCs were observed in the lung by fluorescent microscopy. DiR-labeled hUCB-MNCs were detected in the lung and other organs by bioluminescence images. Results : Medial thickness, RV/S+ LV and AT/ET were significantly improved 4 weeks after hUCB-MNC transplantation compared with those in mice without hUCB-MNC transplantation. CMTMR-positive hUCB-MNCs were observed in the lung 3 hours after transplantation. Bioluminescence signals were detected more strongly in the lung than in other organs for 24 hours after transplantation. Conclusions : The results indicate that hUCB-MNCs are incorporated into the lung early after hUCB-MNC transplantation and improve MCT-induced PH.
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Journal Title |
The Journal of Medical Investigation
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ISSN | 13496867
13431420
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NCID | AA11166929
AA12022913
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Publisher | Faculty of Medicine Tokushima University
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Volume | 64
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Issue | 1-2
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Start Page | 43
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End Page | 49
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Sort Key | 43
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Published Date | 2017-02
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Remark | 内容要旨・審査要旨・論文本文の公開
本論文は,著者Mikio Suganoの学位論文として提出され,学位審査・授与の対象となっている。 |
EDB ID | |
DOI (Published Version) | |
URL ( Publisher's Version ) | |
FullText File | |
language |
eng
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TextVersion |
ETD
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MEXT report number | 乙第2109号
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Diploma Number | 乙医第1757号
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Granted Date | 2019-12-26
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Degree Name |
Doctor of Medical Science
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Grantor |
Tokushima University
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departments |
University Hospital
Medical Sciences
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