ID | 112928 |
Title Alternative | ナローバンドUVBがHeLa細胞とアレルギー性鼻炎モデルラットの鼻粘膜におけるヒスタミンH1受容体遺伝子発現の亢進とアポトーシスの誘導に与える影響
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Author |
Kitamura, Yoshiaki
Tokushima University
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Okamoto, Kentaro
Tokushima University
Sanada, Nanae
Tokushima University
Yamada, Takuya
Tokushima University
Sugiyama, Manabu
Tokushima University
Michinaga, Shotaro
Osaka Ohtani University
Kitayama, Mika
Tokushima University
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Keywords | Allergic rhinitis
apoptosis
histamine H1 receptor gene
narrowband-ultraviolet B
phototherapy
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Content Type |
Thesis or Dissertation
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Description | Narrowband-ultraviolet B (NB-UVB) phototherapy is used for the treatment of atopic dermatitis. Previously, we reported that irradiation with 200 mJ/cm2 of 310 nm NB-UVB suppressed phorbol-12- myristate-13-acetate (PMA)-induced up-regulation of histamine H1 receptor (H1R) gene expression without induction of apoptosis in HeLa cells. However, the effect of NB-UVB irradiation on nasal symptoms is still unclear. Here, we show that low dose irradiation with 310 nm NB-UVB alleviates nasal symptoms in toluene 2,4-diisocyanate (TDI)-sensitized allergy model rats. Irradiation with 310 nm NB-UVB suppressed PMA-induced H1R mRNA up-regulation in HeLa cells dose-dependently at doses of 75-200 mJ/cm2 and reversibly at a dose of 150 mJ/cm2 without induction of apoptosis. While, at doses of more than 200 mJ/cm2, irradiation with 310 nm NB-UVB induced apoptosis. Western blot analysis showed that the suppressive effect of NB-UVB irradiation on H1R gene expression was through the inhibition of ERK phosphorylation. In TDI-sensitized rat, intranasal irradiation with 310 nm NB-UVB at an estimated dose of 100 mJ/cm2 once a day for three days suppressed TDI-induced sneezes and upregulation of H1R mRNA in nasal mucosa without induction of apoptosis. These findings suggest that repeated intranasal irradiation with low dose of NB-UVB could be clinically used as phototherapy of AR.
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Journal Title |
Journal of Pharmacological Sciences
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ISSN | 13478613
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NCID | AA12022662
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Publisher | Elsevier|Japanese Pharmacological Society
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Volume | 138
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Issue | 1
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Start Page | 54
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End Page | 62
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Published Date | 2018-09-10
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Remark | 内容要旨・審査要旨・論文本文の公開
本論文は, 著者Tatsuya Fujiiの学位論文として提出され, 学位審査・授与の対象となっている。 |
Rights | © 2018 The Authors. Production and hosting by Elsevier B.V. on behalf of Japanese Pharmacological Society. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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EDB ID | |
DOI (Published Version) | |
URL ( Publisher's Version ) | |
FullText File | |
language |
eng
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TextVersion |
ETD
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MEXT report number | 甲第3246号
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Diploma Number | 甲医第1395号
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Granted Date | 2018-11-22
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Degree Name |
Doctor of Medical Science
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Grantor |
Tokushima University
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departments |
University Hospital
Medical Sciences
Pharmaceutical Sciences
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