ID | 113298 |
Author |
Hirata, Yohko
Tokushima University
Hosaka, Toshio
Tokushima University
Le, Chung T.K.
Tokushima University
Jambaldorj, Bayasgalan
Tokushima University
Teshigawara, Kiyoshi
Tokushima University
Sakaue, Hiroshi
Tokushima University
Tokushima University Educator and Researcher Directory
KAKEN Search Researchers
Sakai, Tohru
Tokushima University
Tokushima University Educator and Researcher Directory
KAKEN Search Researchers
Yoshimoto, Katsuhiko
Tokushima University
Tokushima University Educator and Researcher Directory
KAKEN Search Researchers
Nakaya, Yutaka
Tokushima University
Tokushima University Educator and Researcher Directory
KAKEN Search Researchers
|
Keywords | Vimentin
GLUT4
IRAP
|
Content Type |
Journal Article
|
Description | Insulin-responsive aminopeptidase (IRAP) and GLUT4 are two major cargo proteins of GLUT4 storage vesicles (GSVs) that are translocated from a postendosomal storage compartment to the plasma membrane (PM) in response to insulin. The cytoplasmic region of IRAP is reportedly involved in retention of GSVs. In this study, vimentin was identified using the cytoplasmic domain of IRAP as bait. The validity of this interaction was confirmed by pull-down assays and immunoprecipitation in 3T3-L1 adipocytes. In addition, it was shown that GLUT4 translocation to the PM by insulin was decreased in vimentin-depleted adipocytes, presumably due to dispersing GSVs away from the cytoskeleton. These findings suggest that the IRAP binding protein, vimentin, plays an important role in retention of GSVs.
|
Journal Title |
Biochemical and Biophysical Research Communications
|
ISSN | 0006291X
|
NCID | AA00564395
|
Publisher | Elsevier
|
Volume | 405
|
Issue | 1
|
Start Page | 96
|
End Page | 101
|
Published Date | 2011-01-07
|
Remark | 本論文は,著者Yohko Hirataの学位論文として提出され,学位審査・授与の対象となっている。
|
EDB ID | |
DOI (Published Version) | |
URL ( Publisher's Version ) | |
FullText File | |
language |
eng
|
TextVersion |
Publisher
|
departments |
Oral Sciences
Medical Sciences
|