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ID 109378
Title Alternative
ラット腎不全初期における心臓リモデリングには心筋タンパク質と脂質の増加が関与している
Author
Kuwahara, Mieko Kureha Corporation|University of Tokushima
Bannai, Kenji Kureha Corporation
Yamato, Hideyuki Kureha Corporation
Keywords
Cardiac remodeling
Chronic kidney disease
FTIR spectroscopy
Uremic toxin
Lipid accumulation
Content Type
Thesis or Dissertation
Description
Chronic kidney disease (CKD) is associated with increased risks of cardiovascular morbidity and mortality. Cardiac remodeling including myocardial fibrosis and hypertrophy is frequently observed in CKD patients. In this study, we investigate the mechanism involved in cardiac hypertrophy associated with CKD using a rat model, by morphological and chemical component changes of the hypertrophic and non-hypertrophic hearts. Sprague–Dawley rats were 4/5 nephrectomized (Nx) at 11 weeks of age and assigned to no treatment and treatment with AST-120,which was reported to affect the cardiac damage, at 18 weeks of age. At 26 weeks of age, the rats were euthanized under anesthesia, and biochemical tests as well as analysis of cardiac condition were performed by histological and spectrophotometric methods. Cardiac hypertrophy and CKD were observed in 4/5 Nx rats even though vascular calcification and myocardial fibrosis were not detected. The increasing myocardial protein was confirmed in hypertrophic hearts by infrared spectroscopy. The absorption of amide I and other protein bands in hypertrophic hearts increased at the same position as in normal cardiac absorption. Infrared spectra also showed that lipid accumulation was also detected in hypertrophic heart. Conversely, the absorptions of protein were obviously reduced in the myocardium of non-hypertrophic heart with CKD compared to that of hypertrophic heart. The lipid associated absorption was also decreased in non-hypertrophic heart. Our results suggest that cardiac remodeling associated with relatively early-stage CKD may be suppressed by reducing increased myocardial protein and ameliorating cardiac lipid load.
Journal Title
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease
ISSN
09254439
NCID
AA10779707
AA11522998
Publisher
Elsevier
Volume
1842
Issue
9
Start Page
1433
End Page
1443
Published Date
2014-05-02
Remark
内容要旨・審査要旨・論文本文の公開:
内容要旨・審査要旨 : LID201505251021.pdf
論文本文 : k2848_fulltext.pdf
本論文は,著者Mieko Kuwaharaの学位論文として提出され, 学位審査・授与の対象となっている。
著者の申請により要約(2015-05-26公開)に替えて論文全文を公開(2020-03-18)
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第2848号
Diploma Number
甲栄第226号
Granted Date
2015-03-23
Degree Name
Doctor of Nutritional Science
Grantor
Tokushima University
departments
Medical Sciences