ID | 113682 |
Author |
Morito, Katsuya
Tokushima University
Shimizu, Ryota
Tokushima University
Kitamura, Nahoko
Kyoto University
Park, Si-Bum
Kyoto University
Kishino, Shigenobu
Kyoto University
Ogawa, Jun
Kyoto University
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Keywords | Peroxisomal β-oxidation
Hydroxy fatty acid
Linoleic acid
Fatty acid metabolism
Gut lactic acid bacteria
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Content Type |
Journal Article
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Description | Microorganisms in animal gut produce unusual fatty acids from the ingested diet. Two types of hydroxy fatty acids (HFAs), 10-hydroxy-cis-12-octadecenoic acid (HYA) and 10-hydroxy-octadecanoic acid (HYB), are linoleic acid (LA) metabolites produced by Lactobacillus plantarum. In this study, we investigated the metabolism of these HFAs in mammalian cells. When Chinese hamster ovary (CHO) cells were cultured with HYA, approximately 50% of the supplemented HYA disappeared from the dish within 24 hours. On the other hand, the amount of HYA that disappeared from the dish of peroxisome (PEX)-deficient CHO cells was lower than 20%. Significant amounts of C2- and C4-chain-shortened metabolites of HYA were detected in culture medium of HYA-supplemented CHO cells, but not in medium of PEX-deficient cells. These results suggested that peroxisomal β-oxidation is involved in the disappearance of HYA. The PEX-dependent disappearance was observed in the experiment with HYB, but not with LA. We also found that HYA treatment up-regulates peroxisomal β-oxidation activity of human gastric MKN74 cells and intestinal Caco-2 cells. These results indicate a possibility that HFAs produced from gut bacteria affect lipid metabolism of host via modulation of peroxisomal β-oxidation activity.
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Journal Title |
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
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ISSN | 13881981
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NCID | AA11522965
AA11300409
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Publisher | Elsevier
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Volume | 1864
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Issue | 11
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Start Page | 1619
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End Page | 1628
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Published Date | 2019-07-24
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Rights | © 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
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DOI (Published Version) | |
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language |
eng
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TextVersion |
Author
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departments |
Pharmaceutical Sciences
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