High-speed, FPGA-based photon-counting fluorometer with high data-gathering efficiency

We have developed a low-cost, high-efficiency fluorometer using a field-programmable gate array and simultaneous detection of photoelectron pulse trains. The fluorometer covers a time span of 64 ns with a resolution of 1.0 ns/channel. Depending on the number of channels, the signal-gathering efficiency was improved by a factor of 100 relative to that of conventional time-correlated single-photon-counting. This is assuming that the fluorescence intensity is moderately high but still requires photon counting. The dead time for building a histogram has been reduced to zero, which means that the upper limit of the repetitive excitation frequency could exceed that determined by the time span. We describe instrumental details and demonstrate the basic performance.

This is an author-created, un-copyedited version of an article published in 'Measurement Science and Technology'. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at https://doi.org/10.1088/1361-6501/aa6b46.