Total for the last 12 months
number of access : ?
number of downloads : ?
ID 118797
Title Alternative
Urinary adiponectin as a biomarker for DKD
Author
Kawano, Tomoharu Tokushima Bunri University
Aiba, Motohiro Tokushima Bunri University
Hashida, Seiichi Ehime University
Colpitts, Tracey Sysmex R&D Center Americas, Inc.
Watanabe, Toshihiro Sysmex R&D Center Americas, Inc.
Keywords
Immune complex transfer enzyme immunoassay
Ultrasensitive immunoassay
Urinary adiponectin
Kidney disease
Biomarker
Content Type
Journal Article
Description
We previously developed two immune complex transfer enzyme immunoassays (ICT-EIA) to measure total adiponectin (T-AN) and high molecular weight adiponectin (H-AN) in urine and have verified their usefulness as biomarkers for diabetic kidney disease. In this study, we developed T-AN and H-AN assays using the sandwich EIA (Sand-EIA). The reactivities of Sand-EIAs were compared with ICT-EIAs by measuring size exclusion chromatography (SEC) fractions of urine and adiponectin standard. As a result, ICT-EIAs showed higher macromolecular specificity. We then analyzed the molecular profile of adiponectin in the urine of 5 patients with different eGFR stages by measuring SEC fractions of urine. The results showed that smaller adiponectin correlated relatively well with eGFR stage. Finally, because SEC is time-consuming, we investigated that the ratio of T-ANs by Sand-EIA and ICT-EIA could be a good indicator of the monomer adiponectin. The ratio was evaluated using 77 urine samples from patients with diabetes and showed a significant decrease at an earlier stage compared with other biomarkers. In conclusion, we demonstrated a new index to estimate monomer adiponectin in urine by using Sand-EIA and ICT-EIA, and urinary monomer adiponectin can be a good early indicator of deterioration of renal function in diabetic patients.
Journal Title
The Journal of Medical Investigation
ISSN
13496867
13431420
NCID
AA11166929
Publisher
Tokushima University Faculty of Medicine
Volume
70
Issue
3-4
Start Page
464
End Page
470
Sort Key
464
Published Date
2023-08
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Medical Sciences
Institute of Advanced Medical Sciences