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ID 117002
Title Alternative
Rac1シグナル伝達経路を介したカンピロバクター・ジェジュニの宿主細胞侵入部位へのLC3動員
New Function of Autophagy in C. jejuni Invasion
Author
Fukushima, Shiho Tokushima University
Shimohata, Takaaki Tokushima University|Fukui Prefectural University KAKEN Search Researchers
Inoue, Yuri Tokushima University
Kido, Junko Tokushima University
Keywords
Campylobacter jejuni
autophagy
LC3
Rac1
invasion
Content Type
Thesis or Dissertation
Description
Campylobacter jejuni is a leading cause of food-borne disease worldwide. The pathogenicity of C. jejuni is closely associated with the internalization process in host epithelial cells, which is related to a host immune response. Autophagy indicates a key role in the innate immune system of the host to exclude invasive pathogens. Most bacteria are captured by autophagosomes and degraded by autophagosome-lysosome fusion in host cells. However, several pathogens, such as Salmonella and Shigella, avoid and/or escape autophagic degradation to establish infection. But autophagy involvement as a host immune response to C. jejuni infection has not been clarified. This study revealed autophagy association in C. jejuni infection. During infection, C. jejuni activated the Rho family small GTPase Rac1 signaling pathway, which modulates actin remodeling and promotes the internalization of this pathogen. In this study, we found the LC3 contribution to C. jejuni invasion signaling via the Rac1 signaling pathway. Interestingly, during C. jejuni invasion, LC3 was recruited to bacterial entry site depending on Rac1 GTPase activation just at the early step of the infection. C. jejuni infection induced LC3-II conversion, and autophagy induction facilitated C. jejuni internalization. Also, autophagy inhibition attenuated C. jejuni invasion step. Moreover, Rac1 recruited LC3 to the cellular membrane, activating the invasion of C. jejuni. Altogether, our findings provide insights into the new function of LC3 in bacterial invasion. We found the interaction between the Rho family small GTPase, Rac1, and autophagy-associated protein, LC3.
Journal Title
Frontiers in Cellular and Infection Microbiology
ISSN
22352988
Publisher
Frontiers Media S.A.
Volume
12
Start Page
829682
Published Date
2022-03-03
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Shiho Fukushimaの学位論文として提出され,学位審査・授与の対象となっている。
Rights
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) (https://creativecommons.org/licenses/by/4.0/). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3601号
Diploma Number
甲栄第289号
Granted Date
2022-03-23
Degree Name
Doctor of Nutritional Science
Grantor
Tokushima University
departments
Medical Sciences