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ID 119387
Title Alternative
Porphyromonas gingivalis感染は細胞外小胞のmicroRNA構成を変化させる
Author
Yoshida, Kayo Tokushima University
Takai, Ayu Tokushima University
Seyama, Mariko Tokushima University
Mekata, Mana Tokushima University
Keywords
periodontal disease
microRNA
macrophage
extracellular vesicle
Porphyromonas gingivalis
Content Type
Thesis or Dissertation
Description
Objectives: Periodontitis, commonly associated with Porphyromonas gingivalis (Pg), involves intricate alterations of oral intercellular interactions, in which extracellular vesicles (EVs) play a pivotal role. The understanding of the miRNA profiles in the EVs derived from Pg-infected cells (Pg-EVs) remains incomplete despite acknowledging their importance in intercellular communication during periodontitis. Therefore, our objective was to identify and characterize the miRNAs enriched in Pg-EVs.
Methods: Microarray analysis was conducted to examine the miRNA profiles in the EVs derived from Pg-infected THP-1 cells. We compared the identified miRNAs with those upregulated in the EVs after stimulation with LPS. Additionally, we explored how inhibiting TLR signaling during Pg infection affects the transcription of specific miRNAs. We investigated the unique sequence motifs specific to the miRNAs concentrated in Pg-EVs.
Results: The levels of eleven miRNAs, including miR-155, were increased in Pg-EVs compared with those elevated after LPS stimulation. The Pg-induced miR-155 upregulation via TLR2 but not TLR4 signaling suggests the influence of TLR signaling on the miRNA composition of EVs. Furthermore, the miRNAs upregulated in Pg-EVs contained AGAGGG and GRGGSGC sequence motifs.
Conclusions: Our findings demonstrate that Pg-induced alterations in EV-containing miRNA composition occur in a TLR4-independent manner. Notably, the concentrated miRNAs in Pg-EVs harbor specific motifs with a high G+C content within their sequences. The upregulation of specific miRNAs in EVs under infectious conditions suggests the influence of both innate immune receptor signals and miRNA sequence characteristics.
Journal Title
Journal of Oral Biosciences
ISSN
13490079
18803865
NCID
AA11896386
Publisher
The Japanese Association for Oral Biology|Elsevier
Volume
66
Issue
2
Start Page
365
End Page
372
Published Date
2024-04-04
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Kayo Yoshidaの学位論文として提出され,学位審査・授与の対象となっている。
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3833号
Diploma Number
甲口保第9号
Granted Date
2024-05-09
Degree Name
Doctor of Oral Health Science
Grantor
Tokushima University
departments
Oral Sciences
Medical Sciences