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ID 118052
Title Alternative
ホメオボックス型転写因子Irx3はWnt5aの発現に関与し象牙芽細胞の増殖と分化に調整する
Author
Narwidina, Anrizandy Tokushima University
Iwata, Kokoro Tokyo Medical and Dental University
Kurogoushi, Rika Tokyo Medical and Dental University
Sugimoto, Asuna Tokyo Medical and Dental University
Yamakawa, Yoshihito Tokushima University
Yoshizaki, Keigo Kyushu University
Fukumoto, Satoshi Kyushu University
Iwamoto, Tsutomu Tokyo Medical and Dental University KAKEN Search Researchers
Keywords
Iroquois homeobox genes
Irx3
Odontoblasts
Cell proliferation
Cell differentiation
Wnt5a
Content Type
Thesis or Dissertation
Description
Iroquois homeobox (Irx) genes are TALE-class homeobox genes that are evolutionarily conserved across species and have multiple critical cellular functions in fundamental tissue development processes. Previous studies have shown that Irxs genes are expressed during tooth development. However, the precise roles of genes in teeth remain unclear. Here, we demonstrated for the first time that Irx3 is an essential molecule for the proliferation and differentiation of odontoblasts. Using cDNA synthesized from postnatal day 1 (P1) tooth germs, we examined the expression of all Irx genes (Irx1-Irx6) by RT-PCR and found that all genes except Irx4 were expressed in the tooth tissue. Irx1-Irx3 a were expressed in the dental epithelial cell line M3H1 cells, while Irx3 and Irx5 were expressed in the dental mesenchymal cell line mDP cells. Only Irx3 was expressed in both undifferentiated cell lines. Immunostaining also revealed the presence of IRX3 in the dental epithelial cells and mesenchymal condensation. Inhibition of endogenous Irx3 by siRNA blocks the proliferation and differentiation of mDP cells. Wnt3a, Wnt5a, and Bmp4 are factors involved in odontoblast differentiation and were highly expressed in mDP cells by quantitative PCR analysis. Interestingly, the expression of Wnt5a (but not Wnt3a or Bmp4) was suppressed by Irx3 siRNA. These results suggest that Irx3 plays an essential role in part through the regulation of Wnt5a expression during odontoblast proliferation and differentiation.
Journal Title
Biochemical and Biophysical Research Communications
ISSN
0006291X
10902104
NCID
AA00564395
AA11542044
Publisher
Elsevier
Volume
650
Start Page
47
End Page
54
Published Date
2023-02-04
Remark
内容要旨・審査要旨・論文本文の公開
本論文は,著者Anrizandy Narwidinaの学位論文として提出され,学位審査・授与の対象となっている。
Rights
This is an open access article under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
ETD
MEXT report number
甲第3748号
Diploma Number
甲口第498号
Granted Date
2023-09-20
Degree Name
Doctor of Dental Science
Grantor
Tokushima University
departments
University Hospital
Oral Sciences